melanotransferrin Antibody (A-3): sc-271006

Melanotransferrin Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects melanotransferrin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Melanotransferrin, also known as p97, belongs to the transferrin family of iron-binding proteins, which includes serum transferrin, lactoferrin, and ovotransferrin, and shows notable expression on melanoma cells. Anti-melanotransferrin antibody (A-3) recognizes a unique structure that enables iron binding, though less efficiently than transferrin. This structural feature suggests specialized roles beyond iron transport, including involvement in cancer progression and neurodegenerative diseases. The gene encoding human melanotransferrin resides on chromosome 3q28-29 and expresses predominantly in membrane-bound or secreted forms. In brain tissue, melanotransferrin may serve protective functions against membrane-lipid peroxidation in Alzheimer′s disease and contribute to intracellular adhesion. Understanding melanotransferrin′s structure and function remains crucial for revealing biological significance and therapeutic potential.

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melanotransferrin Antibody (A-3) References:

1. Expression of melanotransferrin isoforms in human serum: relevance to Alzheimer's disease.  |  Desrosiers, RR., et al. 2003. Biochem J. 374: 463-71. PMID: 12809550
2. Melanotransferrin induces human melanoma SK-Mel-28 cell invasion in vivo.  |  Bertrand, Y., et al. 2007. Biochem Biophys Res Commun. 353: 418-23. PMID: 17196552
3. The melanoma tumor antigen, melanotransferrin (p97): a 25-year hallmark--from iron metabolism to tumorigenesis.  |  Suryo Rahmanto, Y., et al. 2007. Oncogene. 26: 6113-24. PMID: 17452986
4. Pattern of Melanotransferrin Expression in Human Colorectal Tissues: An Immunohistochemical Study on Potential Clinical Application.  |  Duś-Szachniewicz, K., et al. 2015. Anticancer Res. 35: 6551-61. PMID: 26637869
5. Anti-cancer Antibody Trastuzumab-Melanotransferrin Conjugate (BT2111) for the Treatment of Metastatic HER2+ Breast Cancer Tumors in the Brain: an In-Vivo Study.  |  Nounou, MI., et al. 2016. Pharm Res. 33: 2930-2942. PMID: 27528392
6. A peptide derived from melanotransferrin delivers a protein-based interleukin 1 receptor antagonist across the BBB and ameliorates neuropathic pain in a preclinical model.  |  Thom, G., et al. 2019. J Cereb Blood Flow Metab. 39: 2074-2088. PMID: 29845881
7. Complex of human Melanotransferrin and SC57.32 Fab fragment reveals novel interdomain arrangement with ferric N-lobe and open C-lobe.  |  Hayashi, K., et al. 2021. Sci Rep. 11: 566. PMID: 33436675
8. Melanotransferrin is efficiently sorted on the surface of exosomes secreted by melanoma cells.  |  Bonhoure, A., et al. 2021. Melanoma Res. 31: 338-351. PMID: 33965973
9. Melanotransferrin (MELTF, MFI2, CD228) Expression Attenuates Malignant Melanoma Progression in the A375-Luc2 Murine Metastasis Model and Human Patients.  |  Jandova, J. and Wondrak, GT. 2024. J Invest Dermatol. 144: 2820-2823.e6. PMID: 38909843
10. Mystery of Melanotransferrin in Melanoma.  |  Chhabra, G. and Ahmad, N. 2024. J Invest Dermatol. 144: 2619-2622. PMID: 39283284

The preferred melanotransferrin antibody is melanotransferrin Antibody (E-4): sc-271633.