melanotransferrin Antibody (E-4): sc-271633

melanotransferrin Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects melanotransferrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). melanotransferrin Antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Melanotransferrin, also known as p97, is a member of the transferrin family of iron-binding proteins, which includes serum transferrin, lactoferrin, and ovotransferrin, and is notably expressed on melanoma cells. Melanotransferrin plays a crucial role in cellular processes, particularly in the brain, where melanotransferrin may be implicated in neurodegenerative diseases such as Alzheimer′s disease. Melanotransferrin is primarily located in the cell membrane and can also be secreted, which is significant for cellular adhesion and protection against oxidative stress. The gene encoding human melanotransferrin is mapped to chromosome 3q28-29, and melanotransferrin expression in the brain suggests a potential role in neuroprotection and cellular signaling. Additionally, melanotransferrin may exhibit metalloprotease activity, further indicating involvement in various biological processes. Further research is essential to fully understand the diverse functions and interactions of melanotransferrin, making anti-melanotransferrin antibody (E-4) a valuable tool for studying melanotransferrin′s role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

melanotransferrin Antibody (E-4) References:

1. Melanotransferrin is produced by senile plaque-associated reactive microglia in Alzheimer's disease.  |  Yamada, T., et al. 1999. Brain Res. 845: 1-5. PMID: 10529437
2. The role of the membrane-bound tumour antigen, melanotransferrin (p97), in iron uptake by the human malignant melanoma cell.  |  Richardson, DR. 2000. Eur J Biochem. 267: 1290-8. PMID: 10691965
3. The membrane-bound transferrin homologue melanotransferrin: roles other than iron transport?  |  Sekyere, E. and Richardson, DR. 2000. FEBS Lett. 483: 11-6. PMID: 11033347
4. The soluble form of the membrane-bound transferrin homologue, melanotransferrin, inefficiently donates iron to cells via nonspecific internalization and degradation of the protein.  |  Food, MR., et al. 2002. Eur J Biochem. 269: 4435-45. PMID: 12230555
5. High transcytosis of melanotransferrin (P97) across the blood-brain barrier.  |  Demeule, M., et al. 2002. J Neurochem. 83: 924-33. PMID: 12421365
6. The human melanoma associated protein melanotransferrin promotes endothelial cell migration and angiogenesis in vivo.  |  Sala, R., et al. 2002. Eur J Cell Biol. 81: 599-607. PMID: 12494997
7. A molecular model for the tumour-associated antigen, p97, suggests a Zn-binding function.  |  Garratt, RC. and Jhotí, H. 1992. FEBS Lett. 305: 55-61. PMID: 1633859
8. In vivo inhibition of angiogenesis by a soluble form of melanotransferrin.  |  Michaud-Levesque, J., et al. 2007. Carcinogenesis. 28: 280-8. PMID: 16857721
9. Roads to melanoma: Key pathways and emerging players in melanoma progression and oncogenic signaling.  |  Paluncic, J., et al. 2016. Biochim Biophys Acta. 1863: 770-84. PMID: 26844774
10. Knockdown of lncRNA MFI2-AS1 inhibits lipopolysaccharide-induced osteoarthritis progression by miR-130a-3p/TCF4.  |  Luo, X., et al. 2020. Life Sci. 240: 117019. PMID: 31678554
11. Chromosomal sublocalization of the human p97 melanoma antigen.  |  Le Beau, MM., et al. 1986. Hum Genet. 72: 294-6. PMID: 3754536
12. Coincident expression and distribution of melanotransferrin and transferrin receptor in human brain capillary endothelium.  |  Rothenberger, S., et al. 1996. Brain Res. 712: 117-21. PMID: 8705293

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-melanotransferrin Antibody (E-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-melanotransferrin Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

melanotransferrin Antibody (E-4) Specifications

Alternate Product Name: melanotransferrin

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 601-670

Epitope Target: melanotransferrin

Epitope Species: human

Product Reactivity/Detection: melanotransferrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for melanotransferrin Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin