MEK kinase-1 Antibody (2-8B-5E): sc-448
- MEK kinase-1 Antibody (2-8B-5E) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- raised against a 300 amino acid internal region of MEK kinase-1 of human origin
- recommended for detection of MEK kinase-1 of human origin by WB and IP
- available conjugated to agarose for IP
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MEK kinase-1 Antibody (2-8B-5E) for WB applications. These reagents are now offered in bundles with MEK kinase-1 Antibody (2-8B-5E) (see ordering information below).
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MEK kinase-1 Antibody (2-8B-5E) is a mouse monoclonal IgG2b antibody that detects MEK kinase-1 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). MEK kinase-1 plays a crucial role in the mitogen-activated protein (MAP) kinase signaling cascade, which is essential for various cellular processes, including proliferation, differentiation, and survival in response to extracellular stimuli like growth factors. MEK kinase-1 is primarily located in the cytoplasm, where MEK kinase-1 acts as a key regulator that activates downstream MAP kinases, such as ERK and c-Jun NH(2)-terminal kinase (JNK), by phosphorylating MAP2K1 and MAP2K4. Proper functioning of MEK kinase-1 is vital, as dysregulation of this pathway can lead to various diseases, including cancer. Furthermore, MEK kinase-1 is involved in the NFκB signaling pathway, activating central protein kinases like CHUK and IKBKB, which are important for immune response and inflammation. MEK kinase-1 (2-8B-5E) antibody is an invaluable tool for researchers studying these critical signaling pathways and their implications in health and disease.
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MEK kinase-1 Antibody (2-8B-5E) References:
- MEK kinase 1 (MEKK1) transduces c-Jun NH2-terminal kinase activation in response to changes in the microtubule cytoskeleton. | Yujiri, T., et al. 1999. J Biol Chem. 274: 12605-10. PMID: 10212239
- MEK kinase 1 is critically required for c-Jun N-terminal kinase activation by proinflammatory stimuli and growth factor-induced cell migration. | Xia, Y., et al. 2000. Proc Natl Acad Sci U S A. 97: 5243-8. PMID: 10805784
- MEK kinase 1 gene disruption alters cell migration and c-Jun NH2-terminal kinase regulation but does not cause a measurable defect in NF-kappa B activation. | Yujiri, T., et al. 2000. Proc Natl Acad Sci U S A. 97: 7272-7. PMID: 10852963
- Activation of MEK kinase 1 by the c-Abl protein tyrosine kinase in response to DNA damage. | Kharbanda, S., et al. 2000. Mol Cell Biol. 20: 4979-89. PMID: 10866655
- MEK kinase 1 interacts with focal adhesion kinase and regulates insulin receptor substrate-1 expression. | Yujiri, T., et al. 2003. J Biol Chem. 278: 3846-51. PMID: 12458213
- MEK kinase 1 mediates the antiapoptotic effect of the Bcr-Abl oncogene through NF-kappaB activation. | Nawata, R., et al. 2003. Oncogene. 22: 7774-80. PMID: 14586403
- Lysophosphatidic Acid Stimulates Ovarian Cancer Cell Migration via a Ras-MEK Kinase 1 Pathway. | Bian, D., et al. 2004. Cancer Res. 64: 4209-17. PMID: 15205333
- MEK kinase 1 activity is required for definitive erythropoiesis in the mouse fetal liver. | Bonnesen, B., et al. 2005. Blood. 106: 3396-404. PMID: 16081685
- E6201 [(3S,4R,5Z,8S,9S,11E)-14-(ethylamino)-8, 9,16-trihydroxy-3,4-dimethyl-3,4,9,19-tetrahydro-1H-2-benzoxacyclotetradecine-1,7(8H)-dione], a novel kinase inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-1 and MEK kinase-1: in vitro characterization of its anti-inflammatory and antihyperproliferative activities. | Goto, M., et al. 2009. J Pharmacol Exp Ther. 331: 485-95. PMID: 19684251
- Regulation of the activity of MEK kinase 1 (MEKK1) by autophosphorylation within the kinase activation domain. | Deak, JC. and Templeton, DJ. 1997. Biochem J. 322 (Pt 1): 185-92. PMID: 9078260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEK kinase-1 Antibody (2-8B-5E) | sc-448 | 200 µg/ml | $322.00 | |||
MEK kinase-1 Antibody (): m-IgG Fc BP-HRP Bundle | sc-536539 | 200 µg Ab; 10 µg BP | $361.00 | |||
MEK kinase-1 Antibody (): m-IgGκ BP-HRP Bundle | sc-533690 | 200 µg Ab; 40 µg BP | $361.00 | |||
MEK kinase-1 Antibody (): m-IgG2b BP-HRP Bundle | sc-549808 | 200 µg Ab; 10 µg BP | $361.00 | |||
MEK kinase-1 Antibody (2-8B-5E) AC | sc-448 AC | 500 µg/ml, 25% agarose | $424.00 |