MEK-6 Antibody (G-12): sc-166728

MEK-6 Antibody (G-12) is a mouse monoclonal IgG2b antibody that detects MEK-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MEK-6, also known as MKK-6, plays a crucial role in the activation of p38 MAP kinase, which is essential for mediating cellular responses to stress and inflammation. MEK-6 is primarily located in the cytoplasm, where MEK-6 acts as a key regulator in the MAPK signaling pathway, influencing various cellular processes including cell differentiation, apoptosis, and inflammatory response. Proper functioning of MEK-6 is vital, as dysregulation of this pathway can lead to various diseases, including cancer and autoimmune disorders. MEK-6 interacts with several other proteins, including p38 MAP kinase, which MEK-6 activates through phosphorylation, thereby amplifying cellular response to external stimuli. Anti-MEK-6 antibody (G-12) serves as an invaluable tool for researchers studying signaling pathways that govern cellular behavior and implications of pathway dysregulation in disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MEK-6 Antibody (G-12) References:

1. Constitutively active mitogen-activated protein kinase kinase 6 (MKK6) or salicylate induces spontaneous 3T3-L1 adipogenesis.  |  Engelman, JA., et al. 1999. J Biol Chem. 274: 35630-8. PMID: 10585441
2. MEK6 regulates human involucrin gene expression via a p38alpha - and p38delta -dependent mechanism.  |  Dashti, SR., et al. 2001. J Biol Chem. 276: 27214-20. PMID: 11454875
3. Tissue inhibitor of metalloproteinase-1 promotes hematopoietic differentiation via caspase-3 upstream the MEKK1/MEK6/p38alpha pathway.  |  Dassé, E., et al. 2007. Leukemia. 21: 595-603. PMID: 17301822
4. MKK6 phosphorylation regulates production of superoxide by enhancing Rac GTPase activity.  |  Harraz, MM., et al. 2007. Antioxid Redox Signal. 9: 1803-13. PMID: 17854274
5. Differential activation of p38MAPK isoforms by MKK6 and MKK3.  |  Remy, G., et al. 2010. Cell Signal. 22: 660-7. PMID: 20004242
6. MKK6 binds and regulates expression of Parkinson's disease-related protein LRRK2.  |  Hsu, CH., et al. 2010. J Neurochem. 112: 1593-604. PMID: 20067578
7. Differential roles of MAPK kinases MKK3 and MKK6 in osteoclastogenesis and bone loss.  |  Boyle, DL., et al. 2014. PLoS One. 9: e84818. PMID: 24400116
8. MKK6 controls T3-mediated browning of white adipose tissue.  |  Matesanz, N., et al. 2017. Nat Commun. 8: 856. PMID: 29021624
9. Anti‑oncogenic and pro‑myogenic action of the MKK6/p38/AKT axis induced by targeting MEK/ERK in embryonal rhabdomyosarcoma.  |  Di Rocco, A., et al. 2022. Oncol Rep. 48: PMID: 35801577
10. Dissection of MKK6 and p38 Signaling Using Light-Activated Protein Kinases.  |  Rahman, SMT., et al. 2024. Chembiochem. 25: e202300551. PMID: 37856284

The preferred p-MEK-3/6 antibody is p-MEK-3/6 Antibody (B-9): sc-8407.