MEF-2A Antibody (B-4): sc-17785

MEF-2A Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects MEF-2A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MEF-2A antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The myocyte enhancer factor-2 (MEF-2) family of transcription factors plays a crucial role in regulating the development and function of T cells, neuronal cells, and muscle cells, with four family members—MEF-2A, -2B, -2C, and -2D—arising from alternatively spliced transcripts. These proteins bind as homo- and heterodimers to the MEF-2 site in promoter regions of target genes, indicating a functional distinction among them during embryogenesis and development. Notably, MEF-2A is involved in differentiating mesodermal precursor cells into myoblasts, a process that has revealed various tissue-specific factors essential for muscle gene expression. Among these factors are the myogenic basic helix-loop-helix proteins, such as MyoD, myogenin, Myf-5, and MRF4, which, along with the MEF-2 family, bind to specific DNA sequences in regulatory regions of muscle-specific genes, thereby orchestrating complex gene expression patterns necessary for muscle development and function.

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MEF-2A Antibody (B-4) References:

1. Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2.  |  Han, A., et al. 2003. Nature. 422: 730-4. PMID: 12700764
2. KIN-29 SIK regulates chemoreceptor gene expression via an MEF2 transcription factor and a class II HDAC.  |  van der Linden, AM., et al. 2007. EMBO J. 26: 358-70. PMID: 17170704
3. The p38alpha/beta mitogen-activated protein kinases mediate recruitment of CREB-binding protein to preserve fast myosin heavy chain IId/x gene activity in myotubes.  |  Meissner, JD., et al. 2007. J Biol Chem. 282: 7265-75. PMID: 17210568
4. Multiple levels of control of the expression of the human A beta H-J-J locus encoding aspartyl-beta-hydroxylase, junctin, and junctate.  |  Feriotto, G., et al. 2006. Ann N Y Acad Sci. 1091: 184-90. PMID: 17341613
5. Regulation of SRC family coactivators by post-translational modifications.  |  Li, S. and Shang, Y. 2007. Cell Signal. 19: 1101-12. PMID: 17368849
6. Breast cancer associated transcriptional repressor PLU-1/JARID1B interacts directly with histone deacetylases.  |  Barrett, A., et al. 2007. Int J Cancer. 121: 265-75. PMID: 17373667
7. Regulation of myocyte enhancer factor-2 transcription factors by neurotoxins.  |  She, H. and Mao, Z. 2011. Neurotoxicology. 32: 563-6. PMID: 21741404
8. Loss of myocyte enhancer factor 2 expression in osteoclasts leads to opposing skeletal phenotypes.  |  Blixt, N., et al. 2020. Bone. 138: 115466. PMID: 32512162
9. Phosphorylation of the Myogenic Factor Myocyte Enhancer Factor-2 Impacts Myogenesis In Vivo.  |  Vishal, K., et al. 2023. Mol Cell Biol. 43: 241-253. PMID: 37184381
10. The MEF2B homologue differentially expressed in mouse embryonal carcinoma cells.  |  Hidaka, K., et al. 1995. Biochem Biophys Res Commun. 213: 555-60. PMID: 7646512
11. Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23.  |  Hobson, GM., et al. 1995. Genomics. 29: 704-11. PMID: 8575763
12. Regulation of the MEF2 family of transcription factors by p38.  |  Zhao, M., et al. 1999. Mol Cell Biol. 19: 21-30. PMID: 9858528

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEF-2A Antibody (B-4) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-MEF-2A Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEF-2A Antibody (B-4) Specifications

Alternate Product Name: MEF2A

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: MEF-2A

Epitope Species: human

Product Reactivity/Detection: MEF-2A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for MEF-2A Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent