Med7 Antibody (E-4): sc-393210
- Med7 Antibody (E-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 38-233 mapping at the C-terminus of Med7 of human origin
- recommended for detection of Med7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Med7 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Med7 Antibody (E-4) for WB applications. These reagents are now offered in bundles with Med7 Antibody (E-4) (see ordering information below).
Med7 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Med7 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Med7 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Med7 plays a crucial role in transcription regulation within mammalian cells, functioning as a key component of high molecular weight coactivating complexes that facilitate communication between transcriptional activators and RNA polymerase II. Med7 functions as part of the SMCC (SRB and MED protein cofactor complex), which enhances gene-specific activation or repression by DNA-binding transcription factors. Med7′s interaction with Med6 and Srb7 allows for RNAPII holoenzyme assembly, promoting effective transcriptional regulation. Med7′s involvement with coactivator proteins from TRAP and DRIP complexes underscores Med7′s importance in steroid receptor-dependent transcriptional activation, while Med7′s association with positive cofactor 4 (PC4) highlights Med7′s role in repressing basal transcription independently of RNA polymerase II activity. This multifaceted functionality makes Med7 a vital player in gene expression regulation, emphasizing anti-Med7 antibody (E-4)′s significance in research applications aimed at understanding transcriptional mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Med7 Antibody (E-4) References:
- A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. | Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
- Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. | Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
- Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. | Rachez, C., et al. 1999. Nature. 398: 824-8. PMID: 10235266
- A dynamic model for PC4 coactivator function in RNA polymerase II transcription. | Malik, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2192-7. PMID: 9482861
- Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. | Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
- The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. | Xiao, H., et al. 1999. J Biol Chem. 274: 3937-40. PMID: 9933582
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med7 Antibody (E-4) | sc-393210 | 200 µg/ml | $316.00 | |||
Med7 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-530392 | 200 µg Ab; 10 µg BP | $354.00 | |||
Med7 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-523855 | 200 µg Ab, 40 µg BP | $354.00 | |||
Med7 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-544031 | 200 µg Ab; 20 µg BP | $354.00 | |||
Med7 Antibody (E-4) AC | sc-393210 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Med7 Antibody (E-4) HRP | sc-393210 HRP | 200 µg/ml | $316.00 | |||
Med7 Antibody (E-4) FITC | sc-393210 FITC | 200 µg/ml | $330.00 | |||
Med7 Antibody (E-4) PE | sc-393210 PE | 200 µg/ml | $343.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 488 | sc-393210 AF488 | 200 µg/ml | $357.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 546 | sc-393210 AF546 | 200 µg/ml | $357.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 594 | sc-393210 AF594 | 200 µg/ml | $357.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 647 | sc-393210 AF647 | 200 µg/ml | $357.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 680 | sc-393210 AF680 | 200 µg/ml | $357.00 | |||
Med7 Antibody (E-4) Alexa Fluor® 790 | sc-393210 AF790 | 200 µg/ml | $357.00 |