Med15 Antibody (KJ-2): sc-101185

Med15 Antibody (KJ-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Med15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Med15 antibody (KJ-2) is available as a non-conjugated monoclonal isotype antibody. Med15, also known as Mediator of RNA polymerase II transcription subunit 15, ARC105, CTG7A, PCQAP, TIG1, or TNRC7, is a crucial component of the Mediator complex, which serves as a vital link between transcriptional activators and RNA polymerase II, thereby facilitating the regulation of gene expression. This 788 amino acid protein is predominantly localized in the nucleus and cytoplasm, with the highest expression levels found in the placenta and blood, underscoring its importance in various biological processes. Notably, Med15 plays a significant role in lipid homeostasis, a critical aspect of cellular metabolism and energy balance. The gene encoding Med15 is situated on chromosome 22, a region associated with DiGeorge syndrome, indicating that the loss of Med15 may contribute to the pathogenesis of this congenital disorder. Alternative splicing of the Med15 gene results in the production of two isoforms, further highlighting the complexity and regulatory potential of Med15 in cellular functions.

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Med15 Antibody (KJ-2) References:

1. Composite co-activator ARC mediates chromatin-directed transcriptional activation.  |  Näär, AM., et al. 1999. Nature. 398: 828-32. PMID: 10235267
2. A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues.  |  Abraham, S. and Solomon, WB. 2000. Gene. 255: 389-400. PMID: 11024300
3. Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit.  |  Berti, L., et al. 2001. Genomics. 74: 320-32. PMID: 11414760
4. A component of the ARC/Mediator complex required for TGF beta/Nodal signalling.  |  Kato, Y., et al. 2002. Nature. 418: 641-6. PMID: 12167862
5. Methylation of the retinoid response gene TIG1 in prostate cancer correlates with methylation of the retinoic acid receptor beta gene.  |  Zhang, J., et al. 2004. Oncogene. 23: 2241-9. PMID: 14691453
6. An association study of PCQAP polymorphisms and schizophrenia.  |  Sandhu, HK., et al. 2004. Psychiatr Genet. 14: 169-72. PMID: 15318033
7. Silencing of the retinoid response gene TIG1 by promoter hypermethylation in nasopharyngeal carcinoma.  |  Kwong, J., et al. 2005. Int J Cancer. 113: 386-92. PMID: 15455391
8. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.  |  Yang, F., et al. 2006. Nature. 442: 700-4. PMID: 16799563
9. TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system.  |  Ishikawa, H., et al. 2006. FEBS Lett. 580: 4784-92. PMID: 16904669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Med15 Antibody (KJ-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Med15 Antibody (KJ-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Med15 Antibody (KJ-2) Specifications

Alternate Product Name: Med15

Product Clone Name: KJ-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-89

Epitope Target: Med15

Epitope Species: human

Product Reactivity/Detection: Med15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Med15 Antibody (KJ-2): N/A

The preferred Med15 antibody is Med15 Antibody (E-8): sc-518192.