MDM2 Antibody (SMP14): sc-965
- MDM2 Antibody (SMP14) is a mouse monoclonal IgG1 κ MDM2 antibody, cited in 1,224 publications, provided at 200 µg/ml
- raised against amino acids 154-167 of MDM2 of human origin
- MDM2 Antibody (SMP14) is recommended for detection of MDM2, MDM2 p60 cleavage product and p53-MDM2 complexes of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-MDM2 Antibody (SMP14) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- TransCruz reagent for ChIP application (sc-965 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MDM2 Antibody (SMP14) for WB and IHC(P) applications. These reagents are now offered in bundles with MDM2 Antibody (SMP14) (see ordering information below).
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MDM2 Antibody (SMP14) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 154-167 of the human MDM2 protein. Anti-MDM2 monoclonal antibody (SMP14) exhibits cross-reactivity with mouse, rat, and human MDM2, making MDM2 (SMP14) antibody suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry on paraffin-embedded sections (IHCP). MDM2, also known as murine double minute 2, is an E3 ubiquitin-protein ligase that plays a crucial role in regulating the p53 tumor suppressor protein. By binding to p53, MDM2 inhibits its transcriptional activity and promotes its degradation via the ubiquitin-proteasome pathway, thereby maintaining low p53 levels under normal conditions. Overexpression or amplification of the MDM2 gene has been observed in various human cancers, including soft tissue sarcomas, osteosarcomas, and breast tumors, leading to the inactivation of p53′s tumor-suppressive functions and contributing to tumorigenesis. MDM2 monoclonal antibody (SMP14) is an essential tool for cancer research, facilitating the study of MDM2′s role in cancer development and progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MDM2 Antibody (SMP14) References:
- A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. | Kastan, MB., et al. 1992. Cell. 71: 587-97. PMID: 1423616
- Targeting the p53-MDM2 interaction to treat cancer. | Klein, C. and Vassilev, LT. 2004. Br J Cancer. 91: 1415-9. PMID: 15452548
- Acidic domain is indispensable for MDM2 to negatively regulate the acetylation of p53. | Wang, Q., et al. 2008. Biochem Biophys Res Commun. 374: 437-41. PMID: 18638452
- Mdm2 induces mono-ubiquitination of FOXO4. | Brenkman, AB., et al. 2008. PLoS One. 3: e2819. PMID: 18665269
- Participation of p53 protein in the cellular response to DNA damage. | Kastan, MB., et al. 1991. Cancer Res. 51: 6304-11. PMID: 1933891
- Mdm2 as a chromatin modifier. | Wienken, M., et al. 2017. J Mol Cell Biol. 9: 74-80. PMID: 27927750
- Small-molecule MDM2 inhibitors in clinical trials for cancer therapy. | Wang, S. and Chen, FE. 2022. Eur J Med Chem. 236: 114334. PMID: 35429910
- Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides. | Picksley, SM., et al. 1994. Oncogene. 9: 2523-9. PMID: 8058315
- Interactions between p53 and MDM2 in a mammalian cell cycle checkpoint pathway. | Chen, CY., et al. 1994. Proc Natl Acad Sci U S A. 91: 2684-8. PMID: 8146175
- Physical and functional interaction between wild-type p53 and mdm2 proteins. | Haines, DS., et al. 1994. Mol Cell Biol. 14: 1171-8. PMID: 8289798
- Oncoprotein MDM2 conceals the activation domain of tumour suppressor p53. | Oliner, JD., et al. 1993. Nature. 362: 857-60. PMID: 8479525
- Mdm2 promotes the rapid degradation of p53. | Haupt, Y., et al. 1997. Nature. 387: 296-9. PMID: 9153395
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDM2 Antibody (SMP14) | sc-965 | 200 µg/ml | $316.00 | |||
MDM2 Antibody (SMP14): m-IgG Fc BP-HRP Bundle | sc-528121 | 200 µg Ab; 10 µg BP | $354.00 | |||
MDM2 Antibody (SMP14): m-IgGκ BP-HRP Bundle | sc-520424 | 200 µg Ab, 40 µg BP | $354.00 | |||
MDM2 Antibody (SMP14): m-IgG1 BP-HRP Bundle | sc-542773 | 200 µg Ab; 20 µg BP | $354.00 | |||
MDM2 Antibody (SMP14) X | sc-965 X | 200 µg/0.1 ml | $316.00 | |||
MDM2 Antibody (SMP14) AC | sc-965 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MDM2 Antibody (SMP14) HRP | sc-965 HRP | 200 µg/ml | $316.00 | |||
MDM2 Antibody (SMP14) FITC | sc-965 FITC | 200 µg/ml | $330.00 | |||
MDM2 Antibody (SMP14) PE | sc-965 PE | 200 µg/ml | $343.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 488 | sc-965 AF488 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 546 | sc-965 AF546 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 594 | sc-965 AF594 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 647 | sc-965 AF647 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 680 | sc-965 AF680 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) Alexa Fluor® 790 | sc-965 AF790 | 200 µg/ml | $357.00 | |||
MDM2 Antibody (SMP14) B | sc-965 B | 200 µg/ml | $320.00 |