MD-1 Antibody (F-5): sc-390613

MD-1 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MD-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MD-1 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MD-1, also known as lymphocyte antigen 68 and RP105 associated protein, plays a crucial role in regulating cell surface expression of RP105, a molecule originally identified as a B cell surface marker that transmits activation signals leading to B cell proliferation and resistance to apoptosis induced by irradiation. MD-1 is essential for forming lipopolysaccharide (LPS)-signaling complex on B cells, enhancing LPS signaling through Toll-like receptor 4 (TLR4), which is vital for immune response. Structurally, MD-1 consists of 162 amino acids, featuring a predicted 19 amino acid signal peptide and two N-glycosylation sites, which are important for stability and function. MD-1 is highly expressed in B cells, monocytes, and tonsils, and is localized on the cell surface despite lacking a transmembrane region, highlighting a unique role in immune signaling and cellular communication.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MD-1 Antibody (F-5) References:

1. Innate recognition of lipopolysaccharide by Toll-like receptor 4/MD-2 and RP105/MD-1.  |  Miyake, K., et al. 2000. J Endotoxin Res. 6: 389-91. PMID: 11521060
2. Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide.  |  Nagai, Y., et al. 2002. Blood. 99: 1699-705. PMID: 11861286
3. MD-1 is a critical part of the mechanism causing Th1-cytokine-triggered murine fetal loss syndrome.  |  Clark, DA., et al. 2003. Am J Reprod Immunol. 49: 297-307. PMID: 12854734
4. MD-1 expression regulates direct and indirect allorecognition.  |  Hadidi, S. and Gorczynski, RM. 2004. Tissue Antigens. 63: 132-41. PMID: 14705984
5. Inhibition of MD-1 expression by immunosuppressants or antisense oligodeoxynucleotides on skin allograft survival in mice.  |  Zhang, X., et al. 2005. Transplant Proc. 37: 1965-7. PMID: 15919519
6. RP105 is associated with MD-1 and transmits an activation signal in human B cells.  |  Miura, Y., et al. 1998. Blood. 92: 2815-22. PMID: 9763566
7. The functional and structural properties of MD-2 required for lipopolysaccharide binding are absent in MD-1.  |  Tsuneyoshi, N., et al. 2005. J Immunol. 174: 340-4. PMID: 15611257

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MD-1 Antibody (F-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MD-1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MD-1 Antibody (F-5) Specifications

Alternate Product Name: MD-1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 107-158

Epitope Target: MD-1

Epitope Species: human

Product Reactivity/Detection: MD-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MD-1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin