MCT1 Antibody (H-1): sc-365501

MCT1 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCT1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MCT1 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MCT1, also known as Monocarboxylate transporter 1 or SLC16A1, plays a crucial role in cellular metabolism by facilitating the transport of monocarboxylates such as lactate and pyruvate across the plasma membrane. This transport is vital for maintaining cellular energy homeostasis, particularly during anaerobic glycolysis, where lactic acid accumulates and can lead to a decrease in intracellular pH, ultimately inhibiting glycolysis if not efficiently exported. MCT1 is predominantly expressed in tumor cells and erythrocytes, making MCT1 a key player in cancer metabolism and a potential target for therapeutic interventions. MCT1′s structure, characterized by 10-12 transmembrane helices with both the amino and carboxy termini located in the cytoplasm, is essential for function as a proton symporter, allowing selective transport of L-lactate. Understanding MCT1 dynamics and interactions with other proteins in the monocarboxylate transporter family can provide insights into metabolic regulation and the development of metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MCT1 Antibody (H-1) References:

1. Lactate transport in skeletal muscle - role and regulation of the monocarboxylate transporter.  |  Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
2. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.  |  Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
3. Lactate transporters (MCT proteins) in heart and skeletal muscles.  |  Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
4. Abundance and subcellular distribution of MCT1 and MCT4 in heart and fast-twitch skeletal muscles.  |  Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
5. HCAR1/MCT1 Regulates Tumor Ferroptosis through the Lactate-Mediated AMPK-SCD1 Activity and Its Therapeutic Implications.  |  Zhao, Y., et al. 2020. Cell Rep. 33: 108487. PMID: 33296645
6. Partial MCT1 invalidation protects against diet-induced non-alcoholic fatty liver disease and the associated brain dysfunction.  |  Hadjihambi, A., et al. 2023. J Hepatol. 78: 180-190. PMID: 35995127
7. SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle.  |  She, X., et al. 2023. Adv Sci (Weinh). 10: e2301871. PMID: 37541664
8. MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation.  |  Chi, W., et al. 2024. Nat Commun. 15: 163. PMID: 38167945
9. Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats.  |  Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
10. Lactate transport in heart in relation to myocardial ischemia.  |  Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
11. Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.  |  Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
12. Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter.  |  Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCT1 Antibody (H-1) has 100 citations in a variety of scientific publications.

Primary antibodies like Anti-MCT1 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCT1 Antibody (H-1) Specifications

Alternate Product Name: MCT1

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-260

Epitope Target: MCT1

Epitope Species: human

Product Reactivity/Detection: MCT1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 100 citations in various scientific publications

Available Conjugates/Forms for MCT1 Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin