MCM7 Antibody (H-5): sc-374403

MCM7 Antibody (H-5) is a mouse monoclonal IgG2a antibody that detects MCM7 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MCM7, a member of the mini-chromosome maintenance (MCM) protein family, plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once per cell cycle. This is vital for maintaining genomic stability and preventing over-replication, which can lead to genomic instability and cancer. MCM7, along with other MCM proteins, contains an ATP-binding motif that facilitates the opening of double-stranded DNA in an ATP-dependent manner, a key step in the initiation of DNA replication. MCM7 expression peaks during the transition from G1 to S phase of the cell cycle and is influenced by E2F transcription factors and various protein kinases. MCM7 interacts with other MCM proteins to form a complex that exhibits DNA helicase activity, essential for unwinding DNA strands during replication. Anti-MCM7 antibody (H-5) serves as an invaluable tool for researchers studying cell cycle regulation, DNA replication mechanisms, and the implications of MCM proteins in cancer biology.

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MCM7 Antibody (H-5) References:

1. Expression of Mcm7 and Cdc6 in oral squamous cell carcinoma and precancerous lesions.  |  Feng, CJ., et al. 2008. Anticancer Res. 28: 3763-9. PMID: 19189662
2. MCM7 and its hosted miR-25, 93 and 106b cluster elicit YAP/TAZ oncogenic activity in lung cancer.  |  Lo Sardo, F., et al. 2017. Carcinogenesis. 38: 64-75. PMID: 27797825
3. Amplified 7q21-22 gene MCM7 and its intronic miR-25 suppress COL1A2 associated genes to sustain intestinal gastric cancer features.  |  Tamilzhalagan, S., et al. 2017. Mol Carcinog. 56: 1590-1602. PMID: 28059478
4. Discordance of MCM7 mRNA and its Intronic MicroRNA Levels Under Hypoxia.  |  Kondo, H., et al. 2017. Anticancer Res. 37: 3885-3890. PMID: 28668890
5. Pharmacoinformatics and molecular dynamics simulation-based phytochemical screening of neem plant (Azadiractha indica) against human cancer by targeting MCM7 protein.  |  Ahammad, F., et al. 2021. Brief Bioinform. 22: PMID: 33834183
6. Immunohistochemical evaluation of D2-40, Galectin-3, Maspin and MCM7 expression in palate squamous cell carcinomas.  |  Pătru, A., et al. 2021. Rom J Morphol Embryol. 62: 133-149. PMID: 34609416
7. MCM2-7 in Clear Cell Renal Cell Carcinoma: MCM7 Promotes Tumor Cell Proliferation.  |  Zhang, J., et al. 2021. Front Oncol. 11: 782755. PMID: 34993142
8. Bioinformatics approaches identified dasatinib and bortezomib inhibit the activity of MCM7 protein as a potential treatment against human cancer.  |  Samad, A., et al. 2022. Sci Rep. 12: 1539. PMID: 35087187
9. Targeted inhibition of the expression of both MCM5 and MCM7 by miRNA-214 impedes DNA replication and tumorigenesis in hepatocellular carcinoma cells.  |  Wang, J., et al. 2022. Cancer Lett. 539: 215677. PMID: 35490917
10. E2F8 facilitates malignant phenotypes of muscle-invasive bladder cancer via increasing MCM7 expression.  |  Liu, LY., et al. 2025. Biochem Cell Biol. 103: 1-14. PMID: 39601318

The preferred MCM7 antibody is MCM7 Antibody (141.2): sc-9966.