MCM7 Antibody (141.2): sc-9966

MCM7 Antibody (141.2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCM7 protein of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). MCM7 monoclonal antibody (141.2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. The mini-chromosome maintenance (MCM) family of proteins, which includes MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5), and MCM7 (Cdc47), plays a crucial role in regulating DNA replication, ensuring that replication occurs only once during the cell cycle. MCM7 is essential for the formation of the MCM2-7 complex, which acts as a helicase to unwind DNA strands, thereby facilitating the replication process. Proper functioning of MCM7 is vital for maintaining genomic stability, as any dysregulation can lead to uncontrolled cell proliferation and contribute to oncogenesis. Expression of MCM proteins, including MCM7, increases during cell growth, peaking at the transition from the G1 to S phase of the cell cycle, and is tightly regulated by E2F transcription factors and various protein kinases. MCM7′s interaction with other MCM proteins, particularly in the formation of the helicase complex, underscores MCM7′s importance in the DNA replication machinery, making MCM7 (141.2) antibody a valuable tool for studying cell cycle dynamics and the mechanisms of DNA replication.

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MCM7 Antibody (141.2) References:

1. MCM7 as a marker of postsurgical progression in non-functioning pituitary adenomas.  |  Hallén, T., et al. 2021. Eur J Endocrinol. 184: 521-531. PMID: 33524001
2. Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration.  |  Wang, J., et al. 2022. Exp Mol Med. 54: 285-297. PMID: 35332256
3. Phospholipase PLCE1 Promotes Transcription and Phosphorylation of MCM7 to Drive Tumor Progression in Esophageal Cancer.  |  Shi, Q., et al. 2024. Cancer Res. 84: 560-576. PMID: 38117512
4. The splicing factor WBP11 mediates MCM7 intron retention to promote the malignant progression of ovarian cancer.  |  Wei, Y., et al. 2024. Oncogene. 43: 1565-1578. PMID: 38561505
5. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication.  |  Koonin, EV. 1993. Nucleic Acids Res. 21: 2541-7. PMID: 8332451
6. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.  |  Ishimi, Y. 1997. J Biol Chem. 272: 24508-13. PMID: 9305914
7. Protein kinase inhibition in G2 causes mammalian Mcm proteins to reassociate with chromatin and restores ability to replicate.  |  Coverley, D., et al. 1998. Exp Cell Res. 238: 63-9. PMID: 9457057
8. Selective proteolysis of the nuclear replication factor MCM3 in apoptosis.  |  Schwab, BL., et al. 1998. Exp Cell Res. 238: 415-21. PMID: 9473350
9. Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase.  |  Fujita, M., et al. 1998. J Biol Chem. 273: 17095-101. PMID: 9642275
10. E2F3 activity is regulated during the cell cycle and is required for the induction of S phase.  |  Leone, G., et al. 1998. Genes Dev. 12: 2120-30. PMID: 9679057
11. Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation.  |  Zhang, JJ., et al. 1998. EMBO J. 17: 6963-71. PMID: 9843502
12. Human T-cell leukemia virus type 1 tax protein abrogates interleukin-2 dependence in a mouse T-cell line.  |  Iwanaga, Y., et al. 1999. J Virol. 73: 1271-7. PMID: 9882331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCM7 Antibody (141.2) has 195 citations in a variety of scientific publications.

Primary antibodies like Anti-MCM7 Antibody (141.2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCM7 Antibody (141.2) Specifications

Alternate Product Name: MCM7

Product Clone Name: 141.2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MCM7

Epitope Species: human

Product Reactivity/Detection: MCM7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 195 citations in various scientific publications

Available Conjugates/Forms for MCM7 Antibody (141.2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin