Mcl-1 Antibody (8C6D4B1): sc-53951

Mcl-1 Antibody (8C6D4B1) is a mouse monoclonal IgG1 kappa light chain antibody, raised against recombinant human Mcl-1 protein, and is recommended for detecting Mcl-1 from mouse, rat, and human origins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a vital member of the Bcl-2 family of proteins that regulate apoptosis, the programmed cell death essential for development and tissue homeostasis. Structurally, Mcl-1 is characterized by three Bcl-2 homology (BH) domains, BH1, BH2, and BH3, which are crucial for anti-apoptotic function. These domains enable Mcl-1 to interact with pro-apoptotic proteins like Bak and Bax, inhibiting their ability to permeabilize the mitochondrial outer membrane and preventing cytochrome c release, a key step in activating the apoptotic cascade. The structural integrity of these BH domains is essential because alterations can disrupt Mcl-1′s ability to bind targets, affecting cell survival. Additionally, Mcl-1 contains a unique N-terminal region subject to various post-translational modifications, such as phosphorylation and ubiquitination, which regulate stability and turnover. The balance between Mcl-1 synthesis and degradation is critical, as overexpression can lead to apoptosis inhibition, contributing to cancer development and progression by allowing malignant cells to evade death. Understanding Mcl-1 structural nuances provides insights for cancer research and therapeutic development, particularly in designing drugs that can specifically target anti-apoptotic functions. Mcl-1 (8C6D4B1) antibody offers researchers a reliable tool for studying structural and functional aspects of Mcl-1 in various biological contexts.

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.