MaxiKα Antibody (B-1): sc-374142

MaxiKα Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects MaxiKα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MaxiKα (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The KCNMA1 gene, located on chromosome 10q22.3, encodes MaxiKα protein, which is also known as the calcium-activated potassium channel, large conductance calcium- and voltage-dependent potassium channel α subunit, Slo α subunit, and BKCA α subunit. MaxiKα protein plays a crucial role in regulating smooth muscle contraction and neuronal excitability by forming calcium-activated potassium channels, which are essential for maintaining cellular homeostasis. MaxiKα protein is primarily expressed in neurons and smooth muscle tissue, where MaxiKα protein associates with MaxiKβ subunit to create channels that respond to increases in intracellular calcium ion concentrations. The interaction between MaxiKα and MaxiKβ is vital, as this interaction modulates the channel′s sensitivity to calcium, thereby influencing various physiological processes, including muscle relaxation and neurotransmitter release. Additionally, MaxiK channels are implicated in cell shrinkage and apoptosis in pulmonary vascular smooth muscle cells, highlighting their importance in cardiovascular health.

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MaxiKα Antibody (B-1) References:

1. Cloning and characterization of the promoters of the maxiK channel alpha and beta subunits.  |  Dhulipala, PD. and Kotlikoff, MI. 1999. Biochim Biophys Acta. 1444: 254-62. PMID: 10023076
2. Acute activation of Maxi-K channels (hSlo) by estradiol binding to the beta subunit.  |  Valverde, MA., et al. 1999. Science. 285: 1929-31. PMID: 10489376
3. A residue in the intracellular vestibule of the pore is critical for gating and permeation in Ca2+-activated K+ (BKCa) channels.  |  Lippiat, JD., et al. 2000. J Physiol. 529 Pt 1: 131-8. PMID: 11080257
4. Activation of K+ channels induces apoptosis in vascular smooth muscle cells.  |  Krick, S., et al. 2001. Am J Physiol Cell Physiol. 280: C970-9. PMID: 11245614
5. MaxiK channel partners: physiological impact.  |  Lu, R., et al. 2006. J Physiol. 570: 65-72. PMID: 16239267
6. Ca2+- and thromboxane-dependent distribution of MaxiK channels in cultured astrocytes: from microtubules to the plasma membrane.  |  Ou, JW., et al. 2009. Glia. 57: 1280-95. PMID: 19170178
7. The β1-subunit of the MaxiK channel associates with the thromboxane A2 receptor and reduces thromboxane A2 functional effects.  |  Li, M., et al. 2013. J Biol Chem. 288: 3668-77. PMID: 23255603
8. Chronic hypoxia in pregnancy affected vascular tone of renal interlobar arteries in the offspring.  |  Tang, J., et al. 2015. Sci Rep. 5: 9723. PMID: 25983078
9. Overexcited MaxiK and KATP channels underlie obstructive jaundice-induced vasoconstrictor hyporeactivity of arterial smooth muscle.  |  Yuan, YW., et al. 2016. Sci Rep. 6: 39246. PMID: 28000721
10. Cloning and characterization of human and mouse homologs of the Drosophila calcium-activated potassium channel gene, slowpoke.  |  Pallanck, L. and Ganetzky, B. 1994. Hum Mol Genet. 3: 1239-43. PMID: 7987297
11. Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain.  |  Tseng-Crank, J., et al. 1994. Neuron. 13: 1315-30. PMID: 7993625
12. A molecular mechanism for electrical tuning of cochlear hair cells.  |  Ramanathan, K., et al. 1999. Science. 283: 215-7. PMID: 9880252

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MaxiKα Antibody (B-1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MaxiKα Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MaxiKα Antibody (B-1) Specifications

Alternate Product Name: MaxiKα

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 937-1236

Epitope Target: MaxiKα

Epitope Species: human

Product Reactivity/Detection: MaxiKα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MaxiKα Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin