MAVS Antibody (E-6) recommended for detection of MAVS of mouse origin by WB, IP, IF and ELISA

MAVS Antibody (E-6): sc-365334

MAVS Antibody (E-6) is rated 4.6 out of 5 by 7.
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Datasheets

    • MAVS Antibody (E-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping within an N-terminal cytoplasmic domain of MAVS of mouse origin
    • MAVS Antibody (E-6) is recommended for detection of MAVS of mouse origin by WB, IP, IF and ELISA
    • Anti-MAVS Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MAVS (E-6): sc-365334.

Does the MAVS Antibody (E-6): sc-365334 work in BMDMs and MEFs for IF staining? I tried to do IF staining on BMDMs but failed. Any protocol which may work nice?

Asked by: asdzxcx
Thank you for your question. We do not currently have any data in those specific types of mouse cells. However, the antibody is recommended for IF in mouse samples, and we do have data and citations available using this antibody in mouse for this application. Please contact our Technical Service Department for assistance in troubleshooting the antibody.
Answered by: Technical Support
Date published: 2019-05-03

Can MAVS (E-6): sc-365334 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MAVS (E-6): sc-365334 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Great antibody for IP We tried this mouse antibody against MAVS for IP in mouse bone marrow derived macrophages, which works very well.
Date published: 2017-09-17
Rated 5 out of 5 by from strong band for WB analysis Used for WB at a concentration of 1:1000 on mouse kidney extracts. The antibody gives strong band at the expected range of 54-75 kDa .
Date published: 2017-08-12
Rated 5 out of 5 by from Fine antibody It is a good antibody that detects endogenous MAVS in RAW264.7 cells.
Date published: 2017-03-15
Rated 5 out of 5 by from Great choice! This MAVS antibody shows beautiful fluorescent staining with methanol fixed cells.
Date published: 2017-01-31
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-05-02
Rated 3 out of 5 by from Works for IP does not work for WB Works for IP does not work for WB
Date published: 2015-03-26
Rated 5 out of 5 by from Produced positive Western Blot data of MAVS Produced positive Western Blot data of MAVS expression in non-transfected and mouse MAVS transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-10
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