Matriptase Antibody (D-7): sc-365482

Matriptase Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Matriptase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Matriptase antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Matriptase, also known as MT-SP1, ST14, prostamin, and epithin, is a type II transmembrane serine protease that plays a crucial role in the regulation of extracellular matrix remodeling, tumor growth, and metastasis, making Matriptase a significant target in cancer research. Matriptase (D-7) antibody primarily detects expression in the cell membrane, where proteolytic activity is essential for processing various substrates involved in cell signaling and tissue homeostasis. Membrane localization of Matriptase enables precise regulation of proteolytic activity in the extracellular environment, influencing cellular interactions and the tumor microenvironment. Notably, sphingosine 1-phosphate found in serum-derived lipoproteins activates Matriptase, which in turn activates key factors such as urokinase-type plasminogen activator and hepatocyte growth factor. The balance between Matriptase and hepatocyte growth factor activator inhibitor type 1 is critical, as an imbalance can lead to enhanced tumor progression, particularly in advanced-stage ovarian cancers. This highlights Matriptase′s importance in both normal physiological processes and pathological conditions, making anti-Matriptase antibody (D-7) an invaluable tool for researchers studying cancer biology and therapeutic interventions.

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Matriptase Antibody (D-7) References:

1. N-terminal processing is essential for release of epithin, a mouse type II membrane serine protease.  |  Cho, EG., et al. 2001. J Biol Chem. 276: 44581-9. PMID: 11567025
2. Catalytic domain structures of MT-SP1/matriptase, a matrix-degrading transmembrane serine proteinase.  |  Friedrich, R., et al. 2002. J Biol Chem. 277: 2160-8. PMID: 11696548
3. Sphingosine 1-phosphate, present in serum-derived lipoproteins, activates matriptase.  |  Benaud, C., et al. 2002. J Biol Chem. 277: 10539-46. PMID: 11792696
4. Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 1.  |  Denda, K., et al. 2002. J Biol Chem. 277: 14053-9. PMID: 11805118
5. Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching.  |  Ihara, S., et al. 2002. J Biol Chem. 277: 16960-7. PMID: 11864986
6. Expression of the serine protease matriptase and its inhibitor HAI-1 in epithelial ovarian cancer: correlation with clinical outcome and tumor clinicopathological parameters.  |  Oberst, MD., et al. 2002. Clin Cancer Res. 8: 1101-7. PMID: 11948120
7. Matriptase/MT-SP1 is required for postnatal survival, epidermal barrier function, hair follicle development, and thymic homeostasis.  |  List, K., et al. 2002. Oncogene. 21: 3765-79. PMID: 12032844
8. The intracellular seven amino acid motif EEGEVFL is required for matriptase vesicle sorting and translocation to the basolateral plasma membrane.  |  Tseng, CC., et al. 2020. PLoS One. 15: e0228874. PMID: 32049977
9. Role of the polycystic kidney disease domain in matriptase chaperone activity and localization of hepatocyte growth factor activator inhibitor-1.  |  Yamashita, F., et al. 2022. FEBS J. 289: 3422-3439. PMID: 35020274
10. A human anti-matriptase-2 antibody limits iron overload, α-globin aggregates, and splenomegaly in β-thalassemic mice.  |  Wake, M., et al. 2024. Blood Adv. 8: 1898-1907. PMID: 38241484
11. PK/PD investigation of antiviral host matriptase/TMPRSS2 inhibitors in cell models.  |  Gamba, D., et al. 2024. Sci Rep. 14: 16621. PMID: 39025978
12. Use of protease substrate specificity screening in the rational design of selective protease inhibitors with unnatural amino acids: Application to HGFA, matriptase, and hepsin.  |  Mahoney, MW., et al. 2024. Protein Sci. 33: e5110. PMID: 39073183

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Matriptase Antibody (D-7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Matriptase Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Matriptase Antibody (D-7) Specifications

Alternate Product Name: Matriptase

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-350

Epitope Target: Matriptase

Epitope Species: human

Product Reactivity/Detection: Matriptase

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Matriptase Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin