Matriptase-2 Antibody (H-1): sc-514973
- Matriptase-2 Antibody (H-1) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 65-364 mapping within an internal region of Matriptase-2 of human origin
- Matriptase-2 Antibody (H-1) is recommended for detection of Matriptase-2 of human origin by WB, IP, IF and ELISA
- Anti-Matriptase-2 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Matriptase-2 Antibody (H-1) for WB applications. These reagents are now offered in bundles with Matriptase-2 Antibody (H-1) (see ordering information below).
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Matriptase-2 Antibody (H-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Matriptase-2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Matriptase-2 antibody (H-1) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Matriptase-2, also known as TMPRSS6, is a type II transmembrane serine protease that plays a crucial role in liver matrix remodeling and hepcidin expression regulation, vital for systemic iron homeostasis. Matriptase-2 is located on the cell surface, featuring a membrane-anchored extracellular C-terminus and cytoplasmic N-terminus, significant for proteolytic processing and signaling pathways. Matriptase-2 activates various signaling cascades influencing iron metabolism, and dysregulation can lead to iron-refractory iron deficiency anemia (IRIDA). Matriptase-2 interacts with transferrin receptor 1 (TfR1), highlighting importance in iron transport and cellular uptake. Elevated Matriptase-2 expression has been observed in K-RAS G12C type lung adenocarcinoma compared to normal tissue, indicating potential roles in cancer biology. TMPRSS6 gene polymorphisms are associated with IRIDA development, underscoring clinical relevance in hematological disorders. Matriptase-2 suppresses prostate cancer cell migration by modulating epithelial-to-mesenchymal transition, making this protein a significant therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Matriptase-2 Antibody (H-1) References:
- Mutations in TMPRSS6 cause iron-refractory iron deficiency anemia (IRIDA). | Finberg, KE., et al. 2008. Nat Genet. 40: 569-71. PMID: 18408718
- The ectodomain of matriptase-2 plays an important nonproteolytic role in suppressing hepcidin expression in mice. | Enns, CA., et al. 2020. Blood. 136: 989-1001. PMID: 32384154
- Transcriptomic Mapping of Non-Small Cell Lung Cancer K-RAS p.G12C Mutated Tumors: Identification of Surfaceome Targets and Immunologic Correlates. | Alcaraz-Sanabria, A., et al. 2021. Front Immunol. 12: 786069. PMID: 35178045
- Matriptase-2/NR4A3 axis switches TGF-β action toward suppression of prostate cancer cell invasion, tumor growth, and metastasis. | Lin, HY., et al. 2022. Oncogene. 41: 2833-2845. PMID: 35418692
- Functionally impaired isoforms regulate TMPRSS6 proteolytic activity. | Dion, SP., et al. 2022. PLoS One. 17: e0273825. PMID: 36044454
- Associated Effect of SLC40A1 and TMPRSS6 Polymorphisms on Iron Overload. | Duca, L., et al. 2022. Metabolites. 12: PMID: 36295822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Matriptase-2 Antibody (H-1) | sc-514973 | 200 µg/ml | $316.00 | |||
Matriptase-2 Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-524889 | 200 µg Ab, 40 µg BP | $354.00 | |||
Matriptase-2 Antibody (H-1): m-IgG2b BP-HRP Bundle | sc-549195 | 200 µg Ab; 10 µg BP | $354.00 | |||
Matriptase-2 Antibody (H-1) AC | sc-514973 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Matriptase-2 Antibody (H-1) HRP | sc-514973 HRP | 200 µg/ml | $316.00 | |||
Matriptase-2 Antibody (H-1) FITC | sc-514973 FITC | 200 µg/ml | $330.00 | |||
Matriptase-2 Antibody (H-1) PE | sc-514973 PE | 200 µg/ml | $343.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 488 | sc-514973 AF488 | 200 µg/ml | $357.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 546 | sc-514973 AF546 | 200 µg/ml | $357.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 594 | sc-514973 AF594 | 200 µg/ml | $357.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 647 | sc-514973 AF647 | 200 µg/ml | $357.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 680 | sc-514973 AF680 | 200 µg/ml | $357.00 | |||
Matriptase-2 Antibody (H-1) Alexa Fluor® 790 | sc-514973 AF790 | 200 µg/ml | $357.00 |