MAT IIβ Antibody (A-3): sc-390586
- MAT IIβ Antibody (A-3) is a mouse monoclonal IgG2b κ MAT IIβ antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 32-51 of MAT IIβ of human origin
- MAT II beta Antibody (A-3) is recommended for detection of MAT IIβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MAT II beta Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAT IIβ Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with MAT IIβ Antibody (A-3) (see ordering information below).
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MAT IIβ Antibody (A-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects MAT II beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAT IIβ (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Methionine adenosyltransferase (MAT) is crucial for the synthesis of S-adenosylmethionine (AdoMet), a key methyl donor involved in numerous biological processes, including methylation reactions that regulate gene expression and protein function. MAT IIβ, also known as TGR, MAT-II, or SDR23E1, is a 334 amino acid protein that exists as a heterotetramer with two MAT IIα subunits, functioning primarily as a non-catalytic regulatory protein that modulates MAT IIα activity. By altering the kinetic properties of MAT IIα, MAT IIβ enhances susceptibility to inhibition, which is vital for maintaining metabolic homeostasis. This regulatory mechanism is particularly important in hepatoma cells, where MAT IIβ is expressed and may influence cell proliferation by promoting DNA synthesis. Additionally, the presence of multiple isoforms of MAT IIβ, resulting from alternative splicing, underscores the complexity of regulatory roles in various physiological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAT IIβ Antibody (A-3) References:
- Cloning, expression, and functional characterization of the beta regulatory subunit of human methionine adenosyltransferase (MAT II). | LeGros, HL., et al. 2000. J Biol Chem. 275: 2359-66. PMID: 10644686
- Regulation of the human MAT2B gene encoding the regulatory beta subunit of methionine adenosyltransferase, MAT II. | LeGros, L., et al. 2001. J Biol Chem. 276: 24918-24. PMID: 11337507
- Methionine adenosyltransferase II beta subunit gene expression provides a proliferative advantage in human hepatoma. | Martínez-Chantar, ML., et al. 2003. Gastroenterology. 124: 940-8. PMID: 12671891
- Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2beta. | Ramani, K., et al. 2008. Hepatology. 47: 521-31. PMID: 18041713
- Expression pattern, regulation, and functions of methionine adenosyltransferase 2beta splicing variants in hepatoma cells. | Yang, H., et al. 2008. Gastroenterology. 134: 281-91. PMID: 18045590
- Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocelluar carcinoma. | Wang, Q., et al. 2008. World J Gastroenterol. 14: 4633-42. PMID: 18698677
- Selective targeting of leukemic cell growth in vivo and in vitro using a gene silencing approach to diminish S-adenosylmethionine synthesis. | Attia, RR., et al. 2008. J Biol Chem. 283: 30788-95. PMID: 18753136
- Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization. | Okada, G., et al. 1981. Biochemistry. 20: 934-40. PMID: 7213623
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT IIβ Antibody (A-3) | sc-390586 | 200 µg/ml | $316.00 | |||
MAT IIβ Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-530196 | 200 µg Ab; 10 µg BP | $354.00 | |||
MAT IIβ Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-523592 | 200 µg Ab, 40 µg BP | $354.00 | |||
MAT IIβ Antibody (A-3) AC | sc-390586 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MAT IIβ Antibody (A-3) HRP | sc-390586 HRP | 200 µg/ml | $316.00 | |||
MAT IIβ Antibody (A-3) FITC | sc-390586 FITC | 200 µg/ml | $330.00 | |||
MAT IIβ Antibody (A-3) PE | sc-390586 PE | 200 µg/ml | $343.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 488 | sc-390586 AF488 | 200 µg/ml | $357.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 546 | sc-390586 AF546 | 200 µg/ml | $357.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 594 | sc-390586 AF594 | 200 µg/ml | $357.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 647 | sc-390586 AF647 | 200 µg/ml | $357.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 680 | sc-390586 AF680 | 200 µg/ml | $357.00 | |||
MAT IIβ Antibody (A-3) Alexa Fluor® 790 | sc-390586 AF790 | 200 µg/ml | $357.00 | |||
MAT IIβ (A-3) Neutralizing Peptide | sc-390586 P | 100 µg/0.5 ml | $68.00 |