MAT II Antibody (F-12): sc-398917
- MAT II Antibody (F-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 69-92 near the N-terminus of MAT IIα of human origin
- Anti-MAT II Antibody (F-12) is recommended for detection of MAT IIα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MAT II Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAT II Antibody (F-12) for WB applications. These reagents are now offered in bundles with MAT II Antibody (F-12) (see ordering information below).
QUICK LINKS
MAT II Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects MAT IIα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAT II (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Methionine adenosyltransferase (MAT) plays a crucial role in the synthesis of S-adenosylmethionine (AdoMet), a key methyl donor involved in numerous biological processes, including methylation of DNA, proteins, and lipids. MAT is encoded by two distinct genes, MAT1A and MAT2A, which produce liver-specific and non-liver-specific forms of MAT, respectively. The liver-specific MAT I/III isoform is essential for maintaining normal liver function, and MAT I/III inactivation is linked to severe liver diseases such as cirrhosis. Furthermore, MAT1A expression is associated with a differentiated liver cell phenotype, while MAT2A expression indicates a dedifferentiated state, often observed in hepatocellular carcinoma (HCC). Notably, the transition from MAT1A to MAT2A expression in HCC is driven by inflammatory signals such as NF-κB and TNFα, promoting cancer cell proliferation and highlighting MAT IIα′s importance in both liver health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAT II Antibody (F-12) References:
- Role of abnormal methionine metabolism in alcoholic liver injury. | Lu, SC., et al. 2002. Alcohol. 27: 155-62. PMID: 12163143
- S-Adenosylmethionine revisited: its essential role in the regulation of liver function. | Avila, MA., et al. 2002. Alcohol. 27: 163-7. PMID: 12163144
- L-methionine availability regulates expression of the methionine adenosyltransferase 2A gene in human hepatocarcinoma cells: role of S-adenosylmethionine. | Martínez-Chantar, ML., et al. 2003. J Biol Chem. 278: 19885-90. PMID: 12660248
- Induction of human methionine adenosyltransferase 2A expression by tumor necrosis factor alpha. Role of NF-kappa B and AP-1. | Yang, H., et al. 2003. J Biol Chem. 278: 50887-96. PMID: 14530285
- Differential protein expression analysis of Leishmania major reveals novel roles for methionine adenosyltransferase and S-adenosylmethionine in methotrexate resistance. | Drummelsmith, J., et al. 2004. J Biol Chem. 279: 33273-80. PMID: 15190060
- Methionine adenosyltransferase:adrenergic-cAMP mechanism regulates a daily rhythm in pineal expression. | Kim, JS., et al. 2005. J Biol Chem. 280: 677-84. PMID: 15504733
- Silencing of human methionine adenosyltransferase 1A expression by methylation of the coding region. | Li, M., et al. 2015. J Biol Chem. 290: 19541. PMID: 26254270
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT II Antibody (F-12) | sc-398917 | 200 µg/ml | $322.00 | |||
MAT II Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-530968 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAT II Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-524535 | 200 µg Ab, 40 µg BP | $361.00 | |||
MAT II Antibody (F-12): m-IgG2a BP-HRP Bundle | sc-547645 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAT II Antibody (F-12) AC | sc-398917 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MAT II Antibody (F-12) HRP | sc-398917 HRP | 200 µg/ml | $322.00 | |||
MAT II Antibody (F-12) FITC | sc-398917 FITC | 200 µg/ml | $336.00 | |||
MAT II Antibody (F-12) PE | sc-398917 PE | 200 µg/ml | $349.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 488 | sc-398917 AF488 | 200 µg/ml | $364.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 546 | sc-398917 AF546 | 200 µg/ml | $364.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 594 | sc-398917 AF594 | 200 µg/ml | $364.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 647 | sc-398917 AF647 | 200 µg/ml | $364.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 680 | sc-398917 AF680 | 200 µg/ml | $364.00 | |||
MAT II Antibody (F-12) Alexa Fluor® 790 | sc-398917 AF790 | 200 µg/ml | $364.00 | |||
MAT II (F-12) Neutralizing Peptide | sc-398917 P | 100 µg/0.5 ml | $69.00 |