MARCO Antibody (F-3): sc-398053

MARCO Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects MARCO protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-MARCO antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MARCO, known as the macrophage receptor with collagenous structure, plays a crucial role in the immune response by serving as a major receptor on macrophages, particularly in the alveolar region of the lungs. This single-pass type II membrane protein is essential for the recognition and binding of both Gram-positive and Gram-negative bacteria, facilitating their clearance from the body. MARCO expression is significantly elevated during bacterial infections, highlighting its importance in the innate immune defense. Additionally, MARCO is involved in the uptake of silica particles, which can lead to cytotoxic effects in macrophages. Understanding MARCO function is vital, as mutations in the MARCO gene can result in increased pulmonary inflammation and a compromised ability to eliminate pathogens from the lungs, thereby contributing to respiratory diseases.

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MARCO Antibody (F-3) References:

1. Inducible expression of macrophage receptor Marco by dendritic cells following phagocytic uptake of dead cells uncovered by oligonucleotide arrays.  |  Grolleau, A., et al. 2003. J Immunol. 171: 2879-88. PMID: 12960310
2. The scavenger receptor MARCO is required for lung defense against pneumococcal pneumonia and inhaled particles.  |  Arredouani, M., et al. 2004. J Exp Med. 200: 267-72. PMID: 15263032
3. MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages.  |  Arredouani, MS., et al. 2005. J Immunol. 175: 6058-64. PMID: 16237101
4. Disparate regulation and function of the class A scavenger receptors SR-AI/II and MARCO.  |  Józefowski, S., et al. 2005. J Immunol. 175: 8032-41. PMID: 16339540
5. Defective microarchitecture of the spleen marginal zone and impaired response to a thymus-independent type 2 antigen in mice lacking scavenger receptors MARCO and SR-A.  |  Chen, Y., et al. 2005. J Immunol. 175: 8173-80. PMID: 16339556
6. Identification and characterization of murine SCARA5, a novel class A scavenger receptor that is expressed by populations of epithelial cells.  |  Jiang, Y., et al. 2006. J Biol Chem. 281: 11834-45. PMID: 16407294
7. Species-specific restriction of cell surface expression of mouse MARCO glycoprotein in murine cell lines.  |  Kvell, K., et al. 2006. Biochem Biophys Res Commun. 341: 1193-202. PMID: 16460688
8. Role of scavenger receptor MARCO in macrophage responses to CpG oligodeoxynucleotides.  |  Józefowski, S., et al. 2006. J Leukoc Biol. 80: 870-9. PMID: 16882874
9. MARCO mediates silica uptake and toxicity in alveolar macrophages from C57BL/6 mice.  |  Hamilton, RF., et al. 2006. J Biol Chem. 281: 34218-26. PMID: 16984918
10. Superenhancer drives a tumor-specific splicing variant of MARCO to promote triple-negative breast cancer progression.  |  Yang, YS., et al. 2022. Proc Natl Acad Sci U S A. 119: e2207201119. PMID: 36343244
11. Super-enhanced MARCO variant drives triple-negative breast cancer progression.  |  Lin, WC. and Lin, FT. 2022. Proc Natl Acad Sci U S A. 119: e2217953119. PMID: 36469785
12. Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression.  |  Gu, C., et al. 2023. J Immunol. 211: 57-70. PMID: 37212598

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MARCO Antibody (F-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MARCO Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MARCO Antibody (F-3) Specifications

Alternate Product Name: MARCO

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 198-247

Epitope Target: MARCO

Epitope Species: human

Product Reactivity/Detection: MARCO

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MARCO Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MARCO antibody is MARCO Antibody (IBL-12): sc-65353.