LC3A/LC3B Antibody (G-4): sc-398822

MAP LC3α/β Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize both MAP LC3α and MAP LC3β, also known as LC3A and LC3B or MAP1LC3A and MAP1LC3B, respectively. Anti-MAP LC3α/β antibody (G-4) targets an internal epitope spanning amino acids 33-56 of the human MAP LC3β protein, enabling its use in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) across human, mouse, and rat species. MAP LC3α/β plays a pivotal role in the regulation of autophagy, a critical cellular process for maintaining homeostasis by degrading and recycling damaged organelles and proteins. Post-translational modifications, such as the lipidation of MAP LC3β from LC3-I to LC3-II, are essential for its association with autophagosome membranes, facilitating the formation and maturation of autophagosomal vacuoles. Additionally, MAP LC3α exists in two alternatively spliced isoforms that are expressed in tissues including the testis, brain, heart, liver, and skeletal muscle, but are notably absent in the thymus and peripheral blood leukocytes, highlighting its specialized functions in these tissues. MAP LC3β, present in both cytosolic and membrane-bound forms, is primarily expressed in the heart, testis, brain, and skeletal muscle, underscoring its importance in neuronal development and cellular structural integrity. MAP LC3α/β (G-4) antibody is available in both non-conjugated and various conjugated forms, such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® derivatives, providing versatility for diverse experimental setups.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LC3A/LC3B Antibody (G-4) References:

1. Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p.  |  Paz, Y., et al. 2000. J Biol Chem. 275: 25445-50. PMID: 10856287
2. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.  |  Kabeya, Y., et al. 2000. EMBO J. 19: 5720-8. PMID: 11060023
3. The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3.  |  Tanida, I., et al. 2001. J Biol Chem. 276: 1701-6. PMID: 11096062
4. Human microtubule-associated protein 1a (MAP1A) gene: genomic organization, cDNA sequence, and developmental- and tissue-specific expression.  |  Fink, JK., et al. 1996. Genomics. 35: 577-85. PMID: 8812494
5. Gene localization and developmental expression of light chain 3: a common subunit of microtubule-associated protein 1A(MAP1A) and MAP1B.  |  Mann, SS. and Hammarback, JA. 1996. J Neurosci Res. 43: 535-44. PMID: 8833088
6. Microtubule-associated protein 1 light chain 3 is a fibronectin mRNA-binding protein linked to mRNA translation in lamb vascular smooth muscle cells.  |  Zhou, B., et al. 1997. J Clin Invest. 100: 3070-82. PMID: 9399954
7. Microtubule involvement in translational regulation of fibronectin expression by light chain 3 of microtubule-associated protein 1 in vascular smooth muscle cells.  |  Zhou, B. and Rabinovitch, M. 1998. Circ Res. 83: 481-9. PMID: 9734470

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LC3A/LC3B Antibody (G-4) has 101 citations in a variety of scientific publications.

Primary antibodies like Anti-LC3A/LC3B Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LC3A/LC3B Antibody (G-4) Specifications

Alternate Product Name: MAP LC3

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 33-56

Epitope Target: MAP LC3β

Epitope Species: human

Product Reactivity/Detection: MAP LC3α and MAP LC3β of mouse, rat and human origin and MAP LC3β2 of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 101 citations in various scientific publications

Available Conjugates/Forms for LC3A/LC3B Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred LC3B antibody is LC3B Antibody (G-2): sc-271625.