MAL Antibody (E-1): sc-390687

MAL Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects MAL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAL (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAL protein, also known as myelin and lymphocyte protein or T lymphocyte maturation-associated protein, is a nonglycosylated hydrophobic integral membrane protein that plays a crucial role in forming and maintaining myelin sheaths in the nervous system. MAL protein is primarily localized in lipid rafts and apical membranes of epithelial cells, where MAL protein contributes to stabilizing glycosphingolipid-enriched membrane microdomains. This localization is vital for proper sorting and trafficking of oligodendrocytes, which are essential for maintaining the integrity of the central nervous system′s paranodal regions. Additionally, MAL protein is predominantly expressed by oligodendrocytes and Schwann cells during intermediate and late stages of T cell differentiation, highlighting MAL protein′s importance in both neural and immune system functions. MAL protein′s ability to interact with glycosphingolipids facilitates the formation of protein-lipid microdomains in myelin, underscoring MAL protein′s significance in cellular signaling and membrane organization.

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MAL Antibody (E-1) References:

1. Expression of MAL and MAL2, two elements of the protein machinery for raft-mediated transport, in normal and neoplastic human tissue.  |  Marazuela, M. and Alonso, MA. 2004. Histol Histopathol. 19: 925-33. PMID: 15168355
2. Specific downregulation and mistargeting of the lipid raft-associated protein MAL in a glycolipid storage disorder.  |  Saravanan, K., et al. 2004. Neurobiol Dis. 16: 396-406. PMID: 15193296
3. The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system.  |  Schaeren-Wiemers, N., et al. 2004. J Cell Biol. 166: 731-42. PMID: 15337780
4. The SRF target gene Fhl2 antagonizes RhoA/MAL-dependent activation of SRF.  |  Philippar, U., et al. 2004. Mol Cell. 16: 867-80. PMID: 15610731
5. Upregulation of myelin and lymphocyte protein (MAL) after traumatic spinal cord injury in rats.  |  Zhang, J., et al. 2013. J Mol Histol. 44: 125-34. PMID: 23196718
6. Expression of myelin and lymphocyte protein (MAL) in oral carcinogenesis.  |  Pal, SK., et al. 2012. Med Mol Morphol. 45: 222-8. PMID: 23224601
7. The Myelin and Lymphocyte Protein MAL Is Required for Binding and Activity of Clostridium perfringens ε-Toxin.  |  Rumah, KR., et al. 2015. PLoS Pathog. 11: e1004896. PMID: 25993478
8. Differentiation and activation of human CD4 T cells is associated with a gradual loss of myelin and lymphocyte protein.  |  Leitner, J., et al. 2021. Eur J Immunol. 51: 848-863. PMID: 33345332
9. Myelin and lymphocyte protein 2 regulates cell proliferation and metastasis through the Notch pathway in prostate adenocarcinoma.  |  Zheng, C., et al. 2021. Transl Androl Urol. 10: 2067-2077. PMID: 34159087
10. Myelin and lymphocyte protein serves as a prognostic biomarker and is closely associated with the tumor microenvironment in the nephroblastoma.  |  Su, C., et al. 2022. Cancer Med. 11: 1427-1438. PMID: 35023304
11. Identification of new oligodendrocyte- and myelin-specific genes by a differential screening approach.  |  Schaeren-Wiemers, N., et al. 1995. J Neurochem. 65: 10-22. PMID: 7790852
12. Myelin and lymphocyte protein (MAL/MVP17/VIP17) and plasmolipin are members of an extended gene family.  |  Magyar, JP., et al. 1997. Gene. 189: 269-75. PMID: 9168137

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAL Antibody (E-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-MAL Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAL Antibody (E-1) Specifications

Alternate Product Name: MAL

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 61-130

Epitope Target: MAL

Epitope Species: human

Product Reactivity/Detection: MAL

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for MAL Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin