MAGE-D4/MAGE-D4B Antibody (G-12): sc-393059
- MAGE-D4/MAGE-D4B Antibody (G-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 23-310 mapping near the N-terminus of MAGE-D4 of human origin
- Anti-MAGE-D4/MAGE-D4B Antibody (G-12) is recommended for detection of MAGE-D4 and MAGE-D4B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MAGE-D4/MAGE-D4B Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAGE-D4/MAGE-D4B Antibody (G-12) for WB applications. These reagents are now offered in bundles with MAGE-D4/MAGE-D4B Antibody (G-12) (see ordering information below).
MAGE-D4/MAGE-D4B Antibody (G-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects MAGE-D4 and MAGE-D4B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAGE-D4/MAGE-D4B (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAGE-D4 and MAGE-D4B are 741 amino acid proteins encoded by human genes MAGED4 and MAGED4B, respectively, and belong to the MAGE family, which directs the expression of tumor antigens recognized by autologous cytolytic T lymphocytes in human melanomas. MAGE-D4 and MAGE-D4B are specifically expressed in glioma cells among cancer cells, making these proteins glioma-specific members of the MAGE family. In normal tissues, MAGE-D4 and MAGE-D4B are primarily found in brain and ovary, highlighting a potential role in tumor biology. MAGE-D4 and MAGE-D4B undergo post-translational modifications that can influence function and interactions, which may be critical for tumor immunity and development of targeted therapies. While MAGE-D4 and MAGE-D4B are expressed at higher levels in malignant tumors compared to normal tissues, expression is not considered to have prognostic significance, making these proteins intriguing targets for further research in cancer immunotherapy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAGE-D4/MAGE-D4B Antibody (G-12) References:
- MAGE-E1, a new member of the melanoma-associated antigen gene family and its expression in human glioma. | Sasaki, M., et al. 2001. Cancer Res. 61: 4809-14. PMID: 11406556
- Structural characterization and chromosomal localization of the MAGE-E1 gene. | Kawano, Y., et al. 2001. Gene. 277: 129-37. PMID: 11602350
- [Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli]. | Wang, L., et al. 2003. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 19: 148-9. PMID: 15151753
- Contrasting frequencies of antitumor and anti-vaccine T cells in metastases of a melanoma patient vaccinated with a MAGE tumor antigen. | Lurquin, C., et al. 2005. J Exp Med. 201: 249-57. PMID: 15657294
- MAGED4-expression in renal cell carcinoma and identification of an HLA-A*25-restricted MHC class I ligand from solid tumor tissue. | Krämer, BF., et al. 2005. Cancer Biol Ther. 4: 943-8. PMID: 16082191
- Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. | Ito, S., et al. 2006. Lung Cancer. 51: 79-88. PMID: 16225959
- Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. | Chapiro, J., et al. 2006. J Immunol. 176: 1053-61. PMID: 16393993
- [The expression of 11 cancer/testis (CT) antigen genes in esophageal carcinoma]. | Liang, Z., et al. 2005. Zhonghua Zhong Liu Za Zhi. 27: 534-7. PMID: 16438850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAGE-D4/MAGE-D4B Antibody (G-12) | sc-393059 | 200 µg/ml | $322.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-530342 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-523788 | 200 µg Ab, 40 µg BP | $361.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12): m-IgG2a BP-HRP Bundle | sc-547485 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) AC | sc-393059 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) HRP | sc-393059 HRP | 200 µg/ml | $322.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) FITC | sc-393059 FITC | 200 µg/ml | $336.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) PE | sc-393059 PE | 200 µg/ml | $349.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 488 | sc-393059 AF488 | 200 µg/ml | $364.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 546 | sc-393059 AF546 | 200 µg/ml | $364.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 594 | sc-393059 AF594 | 200 µg/ml | $364.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 647 | sc-393059 AF647 | 200 µg/ml | $364.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 680 | sc-393059 AF680 | 200 µg/ml | $364.00 | |||
MAGE-D4/MAGE-D4B Antibody (G-12) Alexa Fluor® 790 | sc-393059 AF790 | 200 µg/ml | $364.00 |