MAdCAM-1 Antibody (H-3): sc-365934

MAdCAM-1 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAdCAM-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAdCAM-1 (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAdCAM-1, or mucosal addressin cell adhesion molecule 1, is a crucial immunoglobulin superfamily adhesion molecule that plays a significant role in the immune system by facilitating lymphocyte homing to mucosal tissues. MAdCAM-1 is primarily expressed on the endothelium of mucosal venules, where MAdCAM-1 interacts with lymphocyte homing receptors such as L-Selectin and Integrin α4/β7, guiding lymphocytes into Peyer′s patches and the intestinal lamina propria. This targeted trafficking is essential for maintaining mucosal immunity and ensuring an effective immune response in the gut. Notably, MAdCAM-1 is upregulated in inflammatory conditions, such as autoimmune-mediated liver disease, highlighting MAdCAM-1′s importance in the pathogenesis of chronic inflammatory disorders. Anti-MAdCAM-1 antibody (H-3)′s ability to detect MAdCAM-1 across multiple species and applications makes this reagent an invaluable tool for researchers studying immune responses and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAdCAM-1 Antibody (H-3) References:

1. Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing coreceptor MAdCAM-1 in specialized endothelial cells of the viscera.  |  Wang, CC., et al. 2000. Dev Biol. 224: 152-67. PMID: 10926756
2. Prevention of a chronic progressive form of experimental autoimmune encephalomyelitis by an antibody against mucosal addressin cell adhesion molecule-1, given early in the course of disease progression.  |  Kanwar, JR., et al. 2000. Immunol Cell Biol. 78: 641-5. PMID: 11114975
3. The microenvironment of human Peyer's patches inhibits the increase in CD38 expression associated with the germinal center reaction.  |  Guilliano, MJ., et al. 2001. J Immunol. 166: 2179-85. PMID: 11160270
4. MAdCAM-1 expressed in chronic inflammatory liver disease supports mucosal lymphocyte adhesion to hepatic endothelium (MAdCAM-1 in chronic inflammatory liver disease).  |  Grant, AJ., et al. 2001. Hepatology. 33: 1065-72. PMID: 11343233
5. Characterization of chemokines and adhesion molecules associated with T cell presence in tertiary lymphoid structures in human lung cancer.  |  de Chaisemartin, L., et al. 2011. Cancer Res. 71: 6391-9. PMID: 21900403
6. Differential Expression of Homing Receptor Ligands on Tumor-Associated Vasculature that Control CD8 Effector T-cell Entry.  |  Woods, AN., et al. 2017. Cancer Immunol Res. 5: 1062-1073. PMID: 29097419
7. β7 integrins contribute to intestinal tumor growth in mice.  |  Das, S., et al. 2018. PLoS One. 13: e0204181. PMID: 30235302
8. Long-term Safety and Efficacy of the Anti-MAdCAM-1 Monoclonal Antibody Ontamalimab [SHP647] for the Treatment of Ulcerative Colitis: The Open-label Study TURANDOT II.  |  Reinisch, W., et al. 2021. J Crohns Colitis. 15: 938-949. PMID: 33599720
9. Novel ferrocenylbisphosphonate hybrid compounds: Synthesis, characterization and potent activity against cancer cell lines.  |  Anusionwu, CG., et al. 2022. Bioorg Med Chem. 58: 116652. PMID: 35180594
10. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo.  |  Hamann, A., et al. 1994. J Immunol. 152: 3282-93. PMID: 7511642
11. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.  |  Berlin, C., et al. 1993. Cell. 74: 185-95. PMID: 7687523
12. Human mucosal addressin cell adhesion molecule-1 is preferentially expressed in intestinal tract and associated lymphoid tissue.  |  Briskin, M., et al. 1997. Am J Pathol. 151: 97-110. PMID: 9212736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAdCAM-1 Antibody (H-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MAdCAM-1 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAdCAM-1 Antibody (H-3) Specifications

Alternate Product Name: MAdCAM-1

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 291-406

Epitope Target: MAdCAM-1

Epitope Species: human

Product Reactivity/Detection: MadCAM-1 (all isoforms)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MAdCAM-1 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin