MAD2B Antibody (F-12): sc-377367
- MAD2B Antibody (F-12) is a mouse monoclonal IgG1 κ MAD2B antibody provided at 200 µg/ml
- raised against amino acids 1-82 mapping at the N-terminus of MAD2B of human origin
- MAD2B Antibody (F-12) is recommended for detection of MAD2B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and bovine
- Anti-MAD2B Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAD2B Antibody (F-12) for WB applications. These reagents are now offered in bundles with MAD2B Antibody (F-12) (see ordering information below).
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MAD2B Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAD2B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD2B (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAD2B plays a crucial role in the mitotic checkpoint, which is essential for ensuring accurate chromosome segregation during cell division. Specifically, MAD2B monitors the attachment of kinetochores to spindle fibers; if this attachment is not properly established, MAD2B delays the onset of anaphase, preventing potential genomic instability. This function is vital as errors in chromosome segregation can lead to aneuploidy, a condition often associated with cancer. Additionally, MAD2B interacts with various proteins involved in cell cycle regulation, further underscoring MAD2B′s importance in maintaining cellular integrity during division. MAD2B′s ability to regulate the timing of anaphase onset highlights MAD2B′s significance in cancer biology, where dysregulation of the cell cycle can contribute to tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAD2B Antibody (F-12) References:
- MAD2B is an inhibitor of the anaphase-promoting complex. | Chen, J. and Fang, G. 2001. Genes Dev. 15: 1765-70. PMID: 11459826
- MAD2B contributes to podocyte injury of diabetic nephropathy via inducing cyclin B1 and Skp2 accumulation. | Su, H., et al. 2015. Am J Physiol Renal Physiol. 308: F728-36. PMID: 25651564
- Rev7/Mad2B plays a critical role in the assembly of a functional mitotic spindle. | Bhat, A., et al. 2015. Cell Cycle. 14: 3929-38. PMID: 26697843
- MAD2B-mediated SnoN downregulation is implicated in fibroblast activation and tubulointerstitial fibrosis. | Tang, H., et al. 2016. Am J Physiol Renal Physiol. 311: F207-16. PMID: 27122545
- MAD2B promotes tubular epithelial-to-mesenchymal transition and renal tubulointerstitial fibrosis via Skp2. | Tang, H., et al. 2016. J Mol Med (Berl). 94: 1297-1307. PMID: 27488450
- Involvement of AMPK in regulating the degradation of MAD2B under high glucose in neuronal cells. | Meng, X., et al. 2017. J Cell Mol Med. 21: 1150-1158. PMID: 27957796
- MAD2B acts as a negative regulatory partner of TCF4 on proliferation in human dermal papilla cells. | Yu, N., et al. 2017. Sci Rep. 7: 11687. PMID: 28916740
- Inhibition of MAD2B alleviates venous neointimal formation by suppressing VSMCs proliferation and migration. | Song, AN., et al. 2021. FASEB J. 35: e21959. PMID: 34605572
- Conditional deletion of MAD2B in forebrain neurons enhances hippocampus-dependent learning and memory in mice. | Cheng, L., et al. 2022. Front Cell Neurosci. 16: 956029. PMID: 36212696
- Inactivation of Mad2B Enhances Apoptosis in Human Cervical Cancer Cell Line upon Cisplatin-Induced DNA Damage. | Kim, JH., et al. 2023. Biomol Ther (Seoul). 31: 340-349. PMID: 36642928
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD2B Antibody (F-12) | sc-377367 | 200 µg/ml | $316.00 | |||
MAD2B Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-530014 | 200 µg Ab; 10 µg BP | $354.00 | |||
MAD2B Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-523298 | 200 µg Ab, 40 µg BP | $354.00 | |||
MAD2B Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-543817 | 200 µg Ab; 20 µg BP | $354.00 | |||
MAD2B Antibody (F-12) AC | sc-377367 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MAD2B Antibody (F-12) HRP | sc-377367 HRP | 200 µg/ml | $316.00 | |||
MAD2B Antibody (F-12) FITC | sc-377367 FITC | 200 µg/ml | $330.00 | |||
MAD2B Antibody (F-12) PE | sc-377367 PE | 200 µg/ml | $343.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 488 | sc-377367 AF488 | 200 µg/ml | $357.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 546 | sc-377367 AF546 | 200 µg/ml | $357.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 594 | sc-377367 AF594 | 200 µg/ml | $357.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 647 | sc-377367 AF647 | 200 µg/ml | $357.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 680 | sc-377367 AF680 | 200 µg/ml | $357.00 | |||
MAD2B Antibody (F-12) Alexa Fluor® 790 | sc-377367 AF790 | 200 µg/ml | $357.00 |