MAD2B Antibody (14): sc-135977

MAD2B Antibody (14) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAD2B in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-MAD2B antibody (14) is available in a non-conjugated form, providing researchers with a versatile tool for studying this critical protein. MAD2B plays a vital role in the regulation of the mitotic spindle assembly checkpoint, ensuring that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. This function is crucial for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to tumorigenesis. MAD2B is localized to unattached kinetochores during pro-metaphase, where MAD2B acts as a sensor for spindle attachment; upon proper attachment, MAD2B disassociates, allowing the cell cycle to progress. Furthermore, MAD2B has been shown to interact with various proteins involved in cell cycle regulation, including the Insulin receptor, which suggests MAD2B′s involvement in mediating specific signaling pathways that influence cell proliferation and survival. Using MAD2B monoclonal antibody (14), researchers can gain insights into the mechanisms of cell cycle control and the implications of MAD2B dysregulation in cancer biology.

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MAD2B Antibody (14) References:

1. Creative blocks: cell-cycle checkpoints and feedback controls.  |  Murray, AW. 1992. Nature. 359: 599-604. PMID: 1406993
2. MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.  |  Ye, C., et al. 2020. Am J Physiol Renal Physiol. 319: F636-F646. PMID: 32830536
3. MAD2B-mediated cell cycle reentry of podocytes is involved in the pathogenesis of FSGS.  |  Bao, D., et al. 2021. Int J Biol Sci. 17: 4396-4408. PMID: 34803506
4. MAD2B promotes podocyte injury through regulating Numb-dependent Notch 1 pathway in diabetic nephropathy.  |  Li, MR., et al. 2022. Int J Biol Sci. 18: 1896-1911. PMID: 35342338
5. MAD2B Blunts Chronic Unpredictable Stress and Corticosterone Stimulation-Induced Depression-Like Behaviors in Mice.  |  Wang, XL., et al. 2023. Int J Neuropsychopharmacol. 26: 137-148. PMID: 36573299
6. The MAD2B-APC/C-MDM2 axis mediates acute kidney injury by modulating p53.  |  Hao, Z., et al. 2023. FEBS J. 290: 3858-3876. PMID: 37002708
7. Mad2B forms a complex with Cdc20, Cdc27, Rev3 and Rev1 in response to cisplatin-induced DNA damage.  |  Kim, JH. and Patel, R. 2023. Korean J Physiol Pharmacol. 27: 427-436. PMID: 37641805
8. Targeting MAD2B as a strategy for ischemic stroke therapy.  |  Zhang, L., et al. 2024. J Adv Res.. PMID: 38972542
9. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores.  |  Chen, RH., et al. 1996. Science. 274: 242-6. PMID: 8824188
10. Identification of a human mitotic checkpoint gene: hsMAD2.  |  Li, Y. and Benezra, R. 1996. Science. 274: 246-8. PMID: 8824189
11. Mitosis: don't get mad, get even.  |  Glotzer, M. 1996. Curr Biol. 6: 1592-4. PMID: 8994822
12. Interaction of MAD2 with the carboxyl terminus of the insulin receptor but not with the IGFIR. Evidence for release from the insulin receptor after activation.  |  O'Neill, TJ., et al. 1997. J Biol Chem. 272: 10035-40. PMID: 9092546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAD2B Antibody (14) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MAD2B Antibody (14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAD2B Antibody (14) Specifications

Alternate Product Name: MAD2B

Product Clone Name: 14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-180

Epitope Target: MAD2B

Epitope Species: human

Product Reactivity/Detection: MAD2B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MAD2B Antibody (14): N/A

The preferred MAD2B antibody is MAD2B Antibody (F-12): sc-377367.