MAD2 Antibody (C-9): sc-393188
- MAD2 Antibody (C-9) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 178-205 at the C-terminus of MAD2 of human origin
- recommended for detection of MAD2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, bovine and porcine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAD2 Antibody (C-9) for WB applications. These reagents are now offered in bundles with MAD2 Antibody (C-9) (see ordering information below).
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MAD2 Antibody (C-9) is a mouse monoclonal IgG1 antibody that detects MAD2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD2 plays a crucial role in the regulation of the mitotic spindle assembly checkpoint, ensuring that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. This function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to tumorigenesis. MAD2 is localized to unattached kinetochores during prometaphase, where MAD2 acts as a sensor for spindle attachment; upon proper attachment, MAD2 disassociates, allowing the cell cycle to progress. Notably, MAD2 has been shown to interact with the insulin receptor, suggesting MAD2′s involvement in insulin signaling pathways, which further underscores MAD2′s importance in cellular processes beyond mitosis. Anti-MAD2 antibody (C-9) is an essential tool for researchers studying cell cycle regulation and its implications in cancer biology, providing insights into the mechanisms that govern cell division and the potential for therapeutic interventions.
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MAD2 Antibody (C-9) References:
- Checkpoint signalling: Mad2 conformers and signal propagation. | Hardwick, KG. 2005. Curr Biol. 15: R122-4. PMID: 15723780
- Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer. | Yang, M., et al. 2008. PLoS Biol. 6: e50. PMID: 18318601
- Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition. | Kim, S., et al. 2010. Proc Natl Acad Sci U S A. 107: 19772-7. PMID: 21041666
- Orderly inactivation of the key checkpoint protein mitotic arrest deficient 2 (MAD2) during mitotic progression. | Ma, HT. and Poon, RY. 2011. J Biol Chem. 286: 13052-9. PMID: 21335556
- Shugoshin is a Mad1/Cdc20-like interactor of Mad2. | Orth, M., et al. 2011. EMBO J. 30: 2868-80. PMID: 21666598
- Mitotic checkpoint proteins Mad1 and Mad2 - structural and functional relationship with implication in genetic diseases. | Avram, S., et al. 2014. Curr Comput Aided Drug Des. 10: 168-81. PMID: 24724894
- Mad2 and BubR1 modulates tumourigenesis and paclitaxel response in MKN45 gastric cancer cells. | Bargiela-Iparraguirre, J., et al. 2014. Cell Cycle. 13: 3590-601. PMID: 25483095
- TRIP13 Functions in the Establishment of the Spindle Assembly Checkpoint by Replenishing O-MAD2. | Ma, HT. and Poon, RYC. 2018. Cell Rep. 22: 1439-1450. PMID: 29425500
- Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. | Alfieri, C., et al. 2018. Nature. 559: 274-278. PMID: 29973720
- Targeting MAD2 modulates stemness and tumorigenesis in human Gastric Cancer cell lines. | Pajuelo-Lozano, N., et al. 2020. Theranostics. 10: 9601-9618. PMID: 32863948
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD2 Antibody (C-9) | sc-393188 | 200 µg/ml | $322.00 | |||
MAD2 Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-538162 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAD2 Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-535722 | 200 µg Ab; 40 µg BP | $361.00 | |||
MAD2 Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-545554 | 200 µg Ab; 20 µg BP | $361.00 | |||
MAD2 (C-9) Neutralizing Peptide | sc-393188 P | 100 µg/0.5 ml | $69.00 |