MAD2 Antibody (17D10): sc-47747

MAD2 Antibody (17D10) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAD2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD2 (17D10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAD2 plays a critical role in the regulation of the cell cycle, specifically at the mitotic spindle assembly checkpoint, where MAD2 ensures proper chromosome segregation. This checkpoint is vital for preventing aneuploidy, a condition that can lead to tumorigenesis. MAD2 is localized to unattached kinetochores during prometaphase, where MAD2 acts as a sensor for spindle fiber attachment; upon successful attachment, MAD2 disassociates, allowing the cell to proceed to anaphase. Notably, cells with mutations in MAD2 fail to arrest during mitosis in response to improper spindle assembly, leading to missegregation of chromosomes and potential cell death. Additionally, research has shown that human MAD2 interacts with the insulin receptor, suggesting MAD2 involvement in insulin signaling pathways, while MAD2B, a homolog of MAD2, is essential for monitoring the kinetochore-spindle attachment process, delaying anaphase onset if the attachment is incomplete. This intricate regulation underscores MAD2 importance in maintaining genomic stability and potential implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MAD2 Antibody (17D10) References:

1. Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis.  |  Liu, ST., et al. 2003. Nat Cell Biol. 5: 341-5. PMID: 12640463
2. Creative blocks: cell-cycle checkpoints and feedback controls.  |  Murray, AW. 1992. Nature. 359: 599-604. PMID: 1406993
3. Complete loss of the tumor suppressor MAD2 causes premature cyclin B degradation and mitotic failure in human somatic cells.  |  Michel, L., et al. 2004. Proc Natl Acad Sci U S A. 101: 4459-64. PMID: 15070740
4. The MAD1 adhesin of Metarhizium anisopliae links adhesion with blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to plants.  |  Wang, C. and St Leger, RJ. 2007. Eukaryot Cell. 6: 808-16. PMID: 17337634
5. Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.  |  Murakami, H., et al. 2007. PLoS One. 2: e317. PMID: 17380189
6. The cell cycle protein MAD2 facilitates endocytosis of the serotonin transporter in the neuronal soma.  |  Koban, F. and Freissmuth, M. 2023. EMBO Rep. 24: e53408. PMID: 37530743
7. MAD2-Dependent Insulin Receptor Endocytosis Regulates Metabolic Homeostasis.  |  Park, J., et al. 2023. Diabetes. 72: 1781-1794. PMID: 37725942
8. MAD2 activates IGF1R/PI3K/AKT pathway and promotes cholangiocarcinoma progression by interfering USP44/LIMA1 complex.  |  Jiang, W., et al. 2023. Oncogene. 42: 3344-3357. PMID: 37752233
9. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores.  |  Chen, RH., et al. 1996. Science. 274: 242-6. PMID: 8824188
10. Identification of a human mitotic checkpoint gene: hsMAD2.  |  Li, Y. and Benezra, R. 1996. Science. 274: 246-8. PMID: 8824189
11. Mitosis: don't get mad, get even.  |  Glotzer, M. 1996. Curr Biol. 6: 1592-4. PMID: 8994822
12. Interaction of MAD2 with the carboxyl terminus of the insulin receptor but not with the IGFIR. Evidence for release from the insulin receptor after activation.  |  O'Neill, TJ., et al. 1997. J Biol Chem. 272: 10035-40. PMID: 9092546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MAD2 Antibody (17D10) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-MAD2 Antibody (17D10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MAD2 Antibody (17D10) Specifications

Alternate Product Name: MAD2L1

Product Clone Name: 17D10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MAD2

Epitope Species: human

Product Reactivity/Detection: MAD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for MAD2 Antibody (17D10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin