MACF1 Antibody (H-12): sc-377533
- MACF1 Antibody (H-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1997-2240 mapping within an internal region of MACF1 of mouse origin
- recommended for detection of MACF1 of mouse origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MACF1 Antibody (H-12) for WB applications. These reagents are now offered in bundles with MACF1 Antibody (H-12) (see ordering information below).
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MACF1 Antibody (H-12) is a mouse monoclonal IgG2a antibody that detects MACF1 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MACF1, or Microtubule-actin cross-linking factor 1, is a crucial 5,327 amino acid protein encoded by the human gene MACF1, belonging to the plakin or cytolinker family. MACF1 features a complex structure that includes one actin-binding domain, two calponin-homology domains, two EF-hand domains, one SH3 domain, and thirty-seven spectrin repeats, which are vital for maintaining cytoskeletal integrity. The spectrin repeats form a three-helix bundle, contributing to MACF1′s ability to cross-link actin filaments with other cytoskeletal components, thereby playing a significant role in cellular architecture and stability. MACF1 is predominantly expressed in the cytoplasm of various tissues, including the lung, brain, spinal cord, skeletal and cardiac muscle, and skin, highlighting MACF1′s importance in diverse cellular functions. MACF1′s ability to interact with both actin and microtubules underscores MACF1′s role in coordinating the cytoskeletal network, which is essential for processes such as cell shape maintenance, motility, and intracellular transport.
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MACF1 Antibody (H-12) References:
- MACF1 Controls Migration and Positioning of Cortical GABAergic Interneurons in Mice. | Ka, M., et al. 2017. Cereb Cortex. 27: 5525-5538. PMID: 27756764
- Deficiency of Macf1 in osterix expressing cells decreases bone formation by Bmp2/Smad/Runx2 pathway. | Qiu, WX., et al. 2020. J Cell Mol Med. 24: 317-327. PMID: 31709715
- MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1. | Su, P., et al. 2020. Biol Open. 9: PMID: 32139394
- Mesenchymal MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation. | Zhao, F., et al. 2020. Cells. 9: PMID: 32143362
- MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm. | Hu, L., et al. 2021. Cell Death Differ. 28: 2160-2178. PMID: 33664480
- MACF1, Involved in the 1p34.2p34.3 Microdeletion Syndrome, is Essential in Cortical Progenitor Polarity and Brain Integrity. | Ka, M., et al. 2022. Cell Mol Neurobiol. 42: 2187-2204. PMID: 33871731
- MACF1 promotes osteoblastic cell migration by regulating MAP1B through the GSK3beta/TCF7 pathway. | Su, P., et al. 2022. Bone. 154: 116238. PMID: 34700040
- The role of MACF1 on acute myeloid leukemia cell proliferation is involved in Runx2-targeted PI3K/Akt signaling. | Wang, P., et al. 2023. Mol Cell Biochem. 478: 433-441. PMID: 35857251
- MACF1 mutations predict poor prognosis: a novel potential therapeutic target for breast cancer. | Tian, Y., et al. 2022. Am J Transl Res. 14: 7670-7688. PMID: 36505342
- THADA, SDHAF4, and MACF1 Gene Polymorphisms and Placental Expression in Women with Gestational Diabetes. | Ustianowski, P., et al. 2022. Genes (Basel). 14: PMID: 36672824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MACF1 Antibody (H-12) | sc-377533 | 200 µg/ml | $322.00 | |||
MACF1 Antibody (H-12): m-IgG Fc BP-HRP Bundle | sc-538051 | 200 µg Ab; 10 µg BP | $361.00 | |||
MACF1 Antibody (H-12): m-IgGκ BP-HRP Bundle | sc-535577 | 200 µg Ab; 40 µg BP | $361.00 | |||
MACF1 Antibody (H-12): m-IgG2a BP-HRP Bundle | sc-548189 | 200 µg Ab; 10 µg BP | $361.00 |