Anti-MACF1 Antibody (A-3) recommended for detection of MACF1 of mouse and rat origin by WB, IP, IF and ELISA

Anti-MACF1 Antibody (A-3): sc-377532

Anti-MACF1 Antibody (A-3) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-MACF1 Antibody (A-3) is a mouse monoclonal IgG1 κ MACF1 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1997-2240 mapping within an internal region of MACF1 of mouse origin
    • Anti-MACF1 Antibody (A-3) is recommended for detection of MACF1 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-MACF1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MACF1 (A-3): sc-377532.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MACF1 Antibody (A-3) for WB applications. These reagents are now offered in bundles with MACF1 Antibody (A-3) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-377532: MACF1 (A-3) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from mouse ES cells use the IP Western and can detect the ladder, clear ladder.
Date published: 2017-04-07
Rated 5 out of 5 by from Produced positive Western blot data of MACF1 Produced positive Western blot data of MACF1 expression in mouse brain tissue extract. -SCBT QC
Date published: 2013-02-11
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