MAAI Antibody (H-4): sc-374404
- MAAI Antibody (H-4) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-216 representing full length MAAI of human origin
- recommended for detection of MAAI of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for MAAI Antibody (H-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with MAAI Antibody (H-4) (see ordering information below).
QUICK LINKS
SEE ALSO...
MAAI Antibody (H-4) is a mouse monoclonal IgG2b antibody that detects maleylacetoacetate isomerase (MAAI) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAAI plays a crucial role in the phenylalanine/phenylacetate degradation pathway by catalyzing the conversion of maleylacetoacetate to fumarylacetoacetate, which is essential for proper amino acid metabolism and prevention of toxic accumulation in the body. Deficiencies in this pathway can lead to serious metabolic disorders such as type I tyrosinemia and phenylketonuria, highlighting MAAI′s importance in maintaining metabolic health. MAAI is predominantly expressed in the liver, with lower expression levels in skeletal muscle and brain, indicating specialized function in hepatic metabolism. The gene encoding MAAI is located on human chromosome 14q24.3, and MAAI exists as a dimer, which may influence enzymatic activity and interactions with other proteins. MAAI exhibits limited activity with known glutathione S-transferase substrates, suggesting a role beyond traditional GST functions, potentially interacting with specific proteins that modulate activity or stability. Anti-MAAI antibody (H-4) is an invaluable tool for researchers studying metabolic pathways and related disorders, providing insights into the functional dynamics of this important enzyme.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAAI Antibody (H-4) References:
- GSTZ1d: a new allele of glutathione transferase zeta and maleylacetoacetate isomerase. | Blackburn, AC., et al. 2001. Pharmacogenetics. 11: 671-8. PMID: 11692075
- Alkylation and inactivation of human glutathione transferase zeta (hGSTZ1-1) by maleylacetone and fumarylacetone. | Lantum, HB., et al. 2002. Chem Res Toxicol. 15: 707-16. PMID: 12018993
- Kinetics of the biotransformation of maleylacetone and chlorofluoroacetic acid by polymorphic variants of human glutathione transferase zeta (hGSTZ1-1). | Lantum, HB., et al. 2002. Chem Res Toxicol. 15: 957-63. PMID: 12119007
- Mass spectral characterization of dichloroacetic acid-modified human glutathione transferase zeta. | Anderson, WB., et al. 2002. Chem Res Toxicol. 15: 1387-97. PMID: 12437329
- Inhibition and recovery of rat hepatic glutathione S-transferase zeta and alteration of tyrosine metabolism following dichloroacetate exposure and withdrawal. | Guo, X., et al. 2006. Drug Metab Dispos. 34: 36-42. PMID: 16199472
- Human glutathione transferase zeta. | Board, PG. and Anders, MW. 2005. Methods Enzymol. 401: 61-77. PMID: 16399379
- Polymorphisms in the human glutathione transferase zeta promoter. | Fang, YY., et al. 2006. Pharmacogenet Genomics. 16: 307-13. PMID: 16609361
- Pharmacogenetic considerations with dichloroacetate dosing. | James, MO. and Stacpoole, PW. 2016. Pharmacogenomics. 17: 743-53. PMID: 27143230
- GSTZ1-1 downregulates Wnt/β-catenin signalling in hepatocellular carcinoma cells. | Lei, C., et al. 2020. FEBS Open Bio. 10: 6-17. PMID: 31782257
- Clinical physiology and pharmacology of GSTZ1/MAAI. | Stacpoole, PW. 2023. Biochem Pharmacol. 217: 115818. PMID: 37742772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAAI Antibody (H-4) | sc-374404 | 200 µg/ml | $322.00 | |||
MAAI Antibody (H-4): m-IgG2b BP-HRP Bundle | sc-548578 | 200 µg Ab; 10 µg BP | $361.00 |