M-Ras Antibody (C-8): sc-518225

M-Ras Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody raised against human M-Ras, a crucial member of the Ras family of GTPases. Anti-M-Ras antibody (C-8) recognizes an epitope within the M-Ras immunogen sequence and demonstrates specificity for human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. M-Ras monoclonal antibody (C-8) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. M-Ras maintains sophisticated spatial organization within cells, residing in both the cytoplasm and plasma membrane where M-Ras orchestrates complex signaling networks. This precise localization proves essential for M-Ras′s ability to interact with various effector proteins and regulate downstream signaling cascades that control cell growth, differentiation, and cytoskeletal dynamics. When M-Ras regulation becomes disrupted, the resulting signaling abnormalities can promote oncogenic transformation. Through these diverse cellular functions, M-Ras (C-8) antibody provides researchers with a valuable tool for investigating both normal signaling mechanisms and cancer biology pathways.

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M-Ras Antibody (C-8) References:

1. M-Ras is activated by bone morphogenetic protein-2 and participates in osteoblastic determination, differentiation, and transdifferentiation.  |  Watanabe-Takano, H., et al. 2010. Exp Cell Res. 316: 477-90. PMID: 19800879
2. M-Ras induces Ral and JNK activation to regulate MEK/ERK-independent gene expression in MCF-7 breast cancer cells.  |  Castro, AF., et al. 2012. J Cell Biochem. 113: 1253-64. PMID: 22121046
3. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants.  |  Trahey, M. and McCormick, F. 1987. Science. 238: 542-5. PMID: 2821624
4. The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product.  |  Calés, C., et al. 1988. Nature. 332: 548-51. PMID: 2833702
5. Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.  |  Adari, H., et al. 1988. Science. 240: 518-21. PMID: 2833817
6. Transient recruitment of M-Ras GTPase to phagocytic cups in RAW264 macrophages during FcγR-mediated phagocytosis.  |  Egami, Y. and Araki, N. 2018. Microscopy (Oxf). 67: 68-74. PMID: 29340604
7. M-Ras/Shoc2 signaling modulates E-cadherin turnover and cell-cell adhesion during collective cell migration.  |  Kota, P., et al. 2019. Proc Natl Acad Sci U S A. 116: 3536-3545. PMID: 30808747
8. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
9. M-Ras is Muscle-Ras, Moderate-Ras, Mineral-Ras, Migration-Ras, and Many More-Ras.  |  Endo, T. 2020. Exp Cell Res. 397: 112342. PMID: 33130177
10. Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus.  |  Shih, TY., et al. 1980. Nature. 287: 686-91. PMID: 6253810
11. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.  |  Ellis, RW., et al. 1981. Nature. 292: 506-11. PMID: 6265801
12. Novel small GTPase M-Ras participates in reorganization of actin cytoskeleton.  |  Matsumoto, K., et al. 1997. Oncogene. 15: 2409-17. PMID: 9395237

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-M-Ras Antibody (C-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-M-Ras Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

M-Ras Antibody (C-8) Specifications

Alternate Product Name: M-Ras

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 166-188

Epitope Target: M-Ras

Epitope Species: human

Product Reactivity/Detection: M-Ras

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for M-Ras Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin