LysRS Antibody (A-10): sc-271331
- LysRS Antibody (A-10) is a mouse monoclonal IgG1 κ LysRS antibody provided at 200 µg/ml
- raised against amino acids 22-321 mapping near the N-terminus of LysRS of human origin
- LysRS Antibody (A-10) is recommended for detection of LysRS of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-LysRS Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LysRS Antibody (A-10) for WB applications. These reagents are now offered in bundles with LysRS Antibody (A-10) (see ordering information below).
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SEE ALSO...
LysRS Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects LysRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LysRS Antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LysRS protein, also known as lysyl-tRNA synthetase, catalyzes the attachment of lysine to its corresponding tRNA, enabling accurate translation of genetic code into functional proteins. This enzyme exists in both mitochondrial and cytoplasmic isoforms, with the mitochondrial form consisting of 625 amino acids and the cytoplasmic form comprising 576 amino acids. Proper functioning of LysRS is vital, as errors in aminoacylation can lead to nonfunctional or misfolded proteins, potentially contributing to autoimmune diseases like polymyositis and dermatomyositis. The gene encoding LysRS is located on human chromosome 16, a region harboring over 900 genes and accounting for nearly 3% of the human genome, highlighting LysRS significance in cellular function and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LysRS Antibody (A-10) References:
- The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript. | Tolkunova, E., et al. 2000. J Biol Chem. 275: 35063-9. PMID: 10952987
- Genomic clustering of tRNA-specific adenosine deaminase ADAT1 and two tRNA synthetases. | Maas, S., et al. 2001. Mamm Genome. 12: 387-93. PMID: 11331948
- Human lysyl-tRNA synthetase is secreted to trigger proinflammatory response. | Park, SG., et al. 2005. Proc Natl Acad Sci U S A. 102: 6356-61. PMID: 15851690
- Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation. | Guo, M., et al. 2008. Proc Natl Acad Sci U S A. 105: 2331-6. PMID: 18272479
- Lysyl-tRNA synthetase is a target for mutant SOD1 toxicity in mitochondria. | Kawamata, H., et al. 2008. J Biol Chem. 283: 28321-8. PMID: 18715867
- Reaction of anti-OJ autoantibodies with components of the multi-enzyme complex of aminoacyl-tRNA synthetases in addition to isoleucyl-tRNA synthetase. | Targoff, IN., et al. 1993. J Clin Invest. 91: 2556-64. PMID: 8514867
- Assignment of two human autoantigen genes-isoleucyl-tRNA synthetase locates to 9q21 and lysyl-tRNA synthetase locates to 16q23-q24. | Nichols, RC., et al. 1996. Genomics. 36: 210-3. PMID: 8812440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LysRS Antibody (A-10) | sc-271331 | 200 µg/ml | $322.00 | |||
LysRS Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-529164 | 200 µg Ab; 10 µg BP | $361.00 | |||
LysRS Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-521912 | 200 µg Ab, 40 µg BP | $361.00 | |||
LysRS Antibody (A-10): m-IgG1 BP-HRP Bundle | sc-543363 | 200 µg Ab; 20 µg BP | $361.00 | |||
LysRS Antibody (A-10) AC | sc-271331 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LysRS Antibody (A-10) HRP | sc-271331 HRP | 200 µg/ml | $322.00 | |||
LysRS Antibody (A-10) FITC | sc-271331 FITC | 200 µg/ml | $336.00 | |||
LysRS Antibody (A-10) PE | sc-271331 PE | 200 µg/ml | $349.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 488 | sc-271331 AF488 | 200 µg/ml | $364.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 546 | sc-271331 AF546 | 200 µg/ml | $364.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 594 | sc-271331 AF594 | 200 µg/ml | $364.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 647 | sc-271331 AF647 | 200 µg/ml | $364.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 680 | sc-271331 AF680 | 200 µg/ml | $364.00 | |||
LysRS Antibody (A-10) Alexa Fluor® 790 | sc-271331 AF790 | 200 µg/ml | $364.00 |