LYPLA3 Antibody (B-2): sc-376078
- LYPLA3 Antibody (B-2) is a mouse monoclonal IgG1 κ LYPLA3 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 246-412 mapping at the C-terminus of LYPLA3 of human origin
- LYPLA3 Antibody (B-2) is recommended for detection of LYPLA3 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-LYPLA3 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LYPLA3 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with LYPLA3 Antibody (B-2) (see ordering information below).
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LYPLA3 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects LYPLA3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). LYPLA3 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LYPLA3, also known as lysophospholipase 3, is crucial for lipid metabolism and is predominantly expressed in the kidney, placenta, pancreas, testis, spleen, heart, and skeletal muscle, where LYPLA3 localizes to lysosomes. LYPLA3 plays a significant role in the degradation of pulmonary surfactant phospholipids, particularly phosphatidylethanolamine and phosphatidylcholine, which are vital for maintaining alveolar stability and function. By transacylating ceramide and increasing lysosomal membrane permeability to potassium ions and protons, LYPLA3 may influence lysosomal osmotic sensitivity, potentially leading to enzyme destabilization and apoptosis. LYPLA3′s ability to remodel acyl groups also suggests involvement in modulating the biological and pharmacological activities of certain lipophilic alcohols, making LYPLA3 an important target for research in lipid metabolism and related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LYPLA3 Antibody (B-2) References:
- Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase. | Hiraoka, M., et al. 2002. J Biol Chem. 277: 10090-9. PMID: 11790796
- Lysosomal phospholipase A2 is selectively expressed in alveolar macrophages. | Abe, A., et al. 2004. J Biol Chem. 279: 42605-11. PMID: 15294901
- Loss of lysophospholipase 3 increases atherosclerosis in apolipoprotein E-deficient mice. | Taniyama, Y., et al. 2005. Biochem Biophys Res Commun. 330: 104-10. PMID: 15781238
- Structure and function of lysosomal phospholipase A2: identification of the catalytic triad and the role of cysteine residues. | Hiraoka, M., et al. 2005. J Lipid Res. 46: 2441-7. PMID: 16106046
- Positional specificity of lysosomal phospholipase A2. | Abe, A., et al. 2006. J Lipid Res. 47: 2268-79. PMID: 16837646
- Lysosomal phospholipase A2 and phospholipidosis. | Hiraoka, M., et al. 2006. Mol Cell Biol. 26: 6139-48. PMID: 16880524
- The phospholipase A2 superfamily and its group numbering system. | Schaloske, RH. and Dennis, EA. 2006. Biochim Biophys Acta. 1761: 1246-59. PMID: 16973413
- Effects of phospholipase A2 on the lysosomal ion permeability and osmotic sensitivity. | Wang, JW., et al. 2006. Chem Phys Lipids. 144: 117-26. PMID: 16982042
- The acylation of lipophilic alcohols by lysosomal phospholipase A2. | Abe, A., et al. 2007. J Lipid Res. 48: 2255-63. PMID: 17626977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LYPLA3 Antibody (B-2) | sc-376078 | 200 µg/ml | $322.00 | |||
LYPLA3 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-529817 | 200 µg Ab; 10 µg BP | $361.00 | |||
LYPLA3 Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-522840 | 200 µg Ab, 40 µg BP | $361.00 | |||
LYPLA3 Antibody (B-2): m-IgG1 BP-HRP Bundle | sc-543705 | 200 µg Ab; 20 µg BP | $361.00 | |||
LYPLA3 Antibody (B-2) AC | sc-376078 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LYPLA3 Antibody (B-2) HRP | sc-376078 HRP | 200 µg/ml | $322.00 | |||
LYPLA3 Antibody (B-2) FITC | sc-376078 FITC | 200 µg/ml | $336.00 | |||
LYPLA3 Antibody (B-2) PE | sc-376078 PE | 200 µg/ml | $349.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 488 | sc-376078 AF488 | 200 µg/ml | $364.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 546 | sc-376078 AF546 | 200 µg/ml | $364.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 594 | sc-376078 AF594 | 200 µg/ml | $364.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 647 | sc-376078 AF647 | 200 µg/ml | $364.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 680 | sc-376078 AF680 | 200 µg/ml | $364.00 | |||
LYPLA3 Antibody (B-2) Alexa Fluor® 790 | sc-376078 AF790 | 200 µg/ml | $364.00 |