LSm5 Antibody (G-5): sc-398656
- LSm5 Antibody (G-5) is a mouse monoclonal IgG1 κ LSm5 antibody provided at 200 µg/ml
- raised against amino acids 1-91 representing full length LSm5 of human origin
- LSm5 Antibody (G-5) is recommended for detection of LSm5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-LSm5 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398656 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LSm5 Antibody (G-5) for WB applications. These reagents are now offered in bundles with LSm5 Antibody (G-5) (see ordering information below).
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LSm5 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects LSm5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LSm5 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LSm5, also known as YER146W, is a 91 amino acid member of the Sm family of proteins, which are crucial for RNA metabolism. These proteins form heptameric complexes that play a significant role in the assembly and function of small nuclear ribonucleoproteins (snRNPs). LSm5 is localized in the nucleus and is essential for the formation of the U6 snRNP, where LSm5 binds to the 3′ end of U6 small nuclear RNA (snRNA) and facilitates the formation of the U4/U6 duplex. This interaction is vital for the splicing process, as LSm5 ensures the proper assembly of the spliceosome, which is necessary for the accurate removal of introns from pre-mRNA. LSm5′s ability to mediate these interactions underscores LSm5′s importance in gene expression regulation and RNA processing, making anti-LSm5 antibody (G-5) a valuable tool for researchers studying RNA metabolism and related cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LSm5 Antibody (G-5) References:
- Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. | Salgado-Garrido, J., et al. 1999. EMBO J. 18: 3451-62. PMID: 10369684
- A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. | Achsel, T., et al. 1999. EMBO J. 18: 5789-802. PMID: 10523320
- Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN). | Friesen, WJ. and Dreyfuss, G. 2000. J Biol Chem. 275: 26370-5. PMID: 10851237
- Autoantibody to hLSm4 and the heptameric LSm complex in anti-Sm sera. | Eystathioy, T., et al. 2002. Arthritis Rheum. 46: 726-34. PMID: 11920408
- The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci. | Ingelfinger, D., et al. 2002. RNA. 8: 1489-501. PMID: 12515382
- A protein interaction framework for human mRNA degradation. | Lehner, B. and Sanderson, CM. 2004. Genome Res. 14: 1315-23. PMID: 15231747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LSm5 Antibody (G-5) | sc-398656 | 200 µg/ml | $322.00 | |||
LSm5 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-530892 | 200 µg Ab; 10 µg BP | $361.00 | |||
LSm5 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-524443 | 200 µg Ab, 40 µg BP | $361.00 | |||
LSm5 Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-544302 | 200 µg Ab; 20 µg BP | $361.00 | |||
LSm5 Antibody (G-5) X | sc-398656 X | 200 µg/0.1 ml | $322.00 | |||
LSm5 Antibody (G-5) AC | sc-398656 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LSm5 Antibody (G-5) HRP | sc-398656 HRP | 200 µg/ml | $322.00 | |||
LSm5 Antibody (G-5) FITC | sc-398656 FITC | 200 µg/ml | $336.00 | |||
LSm5 Antibody (G-5) PE | sc-398656 PE | 200 µg/ml | $349.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 488 | sc-398656 AF488 | 200 µg/ml | $364.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 546 | sc-398656 AF546 | 200 µg/ml | $364.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 594 | sc-398656 AF594 | 200 µg/ml | $364.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 647 | sc-398656 AF647 | 200 µg/ml | $364.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 680 | sc-398656 AF680 | 200 µg/ml | $364.00 | |||
LSm5 Antibody (G-5) Alexa Fluor® 790 | sc-398656 AF790 | 200 µg/ml | $364.00 |