LPCAT1 Antibody (F-9): sc-518182
- LPCAT1 Antibody (F-9) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 174-196 of LPCAT1 of human origin
- recommended for detection of LPCAT1 of human origin by WB, IP, IF and ELISA
- Anti-LPCAT1 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for LPCAT1 Antibody (F-9) for WB applications. This reagent is now offered in a bundle with LPCAT1 Antibody (F-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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LPCAT1 Antibody (F-9) is a mouse monoclonal IgM antibody that detects LPCAT1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LPCAT1 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LPCAT1, or lysophosphatidylcholine acyltransferase 1, is a crucial 534 amino acid single-pass type II membrane protein that plays a significant role in lipid metabolism, particularly in the synthesis of phosphatidylcholine, a key component of cell membranes and pulmonary surfactant. LPCAT1 is primarily located in the endoplasmic reticulum, where LPCAT1 facilitates the conversion of lysophosphatidylcholine into phosphatidylcholine, a process vital for maintaining membrane integrity and function. LPCAT1 is essential for respiratory physiology, contributing to surfactant formation, which reduces surface tension in the lungs and prevents alveolar collapse. Additionally, LPCAT1 is characterized by two EF-hand domains and an HXXXXD motif, which is critical for acyltransferase activity, highlighting LPCAT1′s importance in lipid homeostasis and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LPCAT1 Antibody (F-9) References:
- Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production. | Nakanishi, H., et al. 2006. J Biol Chem. 281: 20140-7. PMID: 16704971
- Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells. | Chen, X., et al. 2006. Proc Natl Acad Sci U S A. 103: 11724-9. PMID: 16864775
- Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes. | Soupene, E., et al. 2008. Proc Natl Acad Sci U S A. 105: 88-93. PMID: 18156367
- Lysophosphatidylcholine acyltransferase 1 (LPCAT1) overexpression in human colorectal cancer. | Mansilla, F., et al. 2009. J Mol Med (Berl). 87: 85-97. PMID: 18974965
- Biosynthesis of phosphatidylcholine by human lysophosphatidylcholine acyltransferase 1. | Harayama, T., et al. 2009. J Lipid Res. 50: 1824-31. PMID: 19383981
- LPCAT1 regulates surfactant phospholipid synthesis and is required for transitioning to air breathing in mice. | Bridges, JP., et al. 2010. J Clin Invest. 120: 1736-48. PMID: 20407208
- Human lysophosphatidylcholine acyltransferases 1 and 2 are located in lipid droplets where they catalyze the formation of phosphatidylcholine. | Moessinger, C., et al. 2011. J Biol Chem. 286: 21330-9. PMID: 21498505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LPCAT1 Antibody (F-9) | sc-518182 | 200 µg/ml | $322.00 | |||
LPCAT1 Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-550805 | 200 µg Ab; 40 µg BP | $361.00 | |||
LPCAT1 Antibody (F-9) AC | sc-518182 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LPCAT1 Antibody (F-9) HRP | sc-518182 HRP | 200 µg/ml | $322.00 | |||
LPCAT1 Antibody (F-9) FITC | sc-518182 FITC | 200 µg/ml | $336.00 | |||
LPCAT1 Antibody (F-9) PE | sc-518182 PE | 200 µg/ml | $349.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 488 | sc-518182 AF488 | 200 µg/ml | $364.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 546 | sc-518182 AF546 | 200 µg/ml | $364.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 594 | sc-518182 AF594 | 200 µg/ml | $364.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 647 | sc-518182 AF647 | 200 µg/ml | $364.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 680 | sc-518182 AF680 | 200 µg/ml | $364.00 | |||
LPCAT1 Antibody (F-9) Alexa Fluor® 790 | sc-518182 AF790 | 200 µg/ml | $364.00 |