LIP5 Antibody (D-6): sc-374012
- LIP5 Antibody (D-6) is a mouse monoclonal IgG1 κ LIP5 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of LIP5 of human origin
- LIP5 Antibody (D-6) is recommended for detection of LIP5 of human origin and Vta1 of mouse and rat origin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-LIP5 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LIP5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with LIP5 Antibody (D-6) (see ordering information below).
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SEE ALSO...
LIP5 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects LIP5 in human, mouse, and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LIP5 Antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIP5, also known as LYST-interacting protein 5, Vps20-associated 1 homolog, dopamine-responsive protein DRG-1, SKD1 binding protein 1, or My012, is a 307 amino acid protein that plays a crucial role in the endosomal multivesicular bodies (MVB) pathway, which is essential for sorting membrane proteins, including lysosomal enzymes, lipids, and stimulated growth factor receptors, for lysosomal degradation. The proper functioning of LIP5 is vital for maintaining cellular homeostasis and regulating protein degradation, which can impact various cellular processes, including immune responses and viral budding. LIP5 is predominantly localized in the cytoplasm and associated with both endosomal and peripheral membranes, and LIP5 expression has been observed in key organs such as the brain, liver, heart, and kidney. RNA interference studies have shown that LIP5 depletion significantly reduces human immunodeficiency virus type 1 (HIV-1) budding, highlighting LIP5′s importance in viral pathogenesis. Additionally, LIP5 interacts with several proteins, including CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1), and potentially CHMP3, indicating LIP5′s involvement in complex protein networks that regulate cellular trafficking and degradation pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LIP5 Antibody (D-6) References:
- Identification of dopamine responsive mRNAs in glial cells by suppression subtractive hybridization. | Shi, J., et al. 2001. Brain Res. 910: 29-37. PMID: 11489251
- Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B. | Fujita, H., et al. 2004. J Cell Sci. 117: 2997-3009. PMID: 15173323
- The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. | Ward, DM., et al. 2005. J Biol Chem. 280: 10548-55. PMID: 15644320
- Ultrastructural analysis of ESCRT proteins suggests a role for endosome-associated tubular-vesicular membranes in ESCRT function. | Welsch, S., et al. 2006. Traffic. 7: 1551-66. PMID: 17014699
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LIP5 Antibody (D-6) | sc-374012 | 200 µg/ml | $322.00 | |||
LIP5 Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-529633 | 200 µg Ab; 10 µg BP | $361.00 | |||
LIP5 Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-522587 | 200 µg Ab, 40 µg BP | $361.00 | |||
LIP5 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-543614 | 200 µg Ab; 20 µg BP | $361.00 | |||
LIP5 Antibody (D-6) AC | sc-374012 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LIP5 Antibody (D-6) HRP | sc-374012 HRP | 200 µg/ml | $322.00 | |||
LIP5 Antibody (D-6) FITC | sc-374012 FITC | 200 µg/ml | $336.00 | |||
LIP5 Antibody (D-6) PE | sc-374012 PE | 200 µg/ml | $349.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 488 | sc-374012 AF488 | 200 µg/ml | $364.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 546 | sc-374012 AF546 | 200 µg/ml | $364.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 594 | sc-374012 AF594 | 200 µg/ml | $364.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 647 | sc-374012 AF647 | 200 µg/ml | $364.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 680 | sc-374012 AF680 | 200 µg/ml | $364.00 | |||
LIP5 Antibody (D-6) Alexa Fluor® 790 | sc-374012 AF790 | 200 µg/ml | $364.00 |