LIMK-1 Antibody (C-10): sc-28370

LIMK-1 Antibody (C-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects LIMK-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LIMK-1 (C-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIMK-1, a member of the LIM kinase family, plays a crucial role in regulating the actin cytoskeleton by phosphorylating and inactivating Cofilin, a protein that severs actin filaments. This regulation is vital for maintaining cellular structure and motility, as LIMK-1 influences processes such as cell migration, morphogenesis, and neuronal differentiation. LIMK-1′s ability to modulate actin dynamics is particularly important in developmental biology and cancer metastasis, where altered cell movement can lead to significant physiological changes. Expression patterns of LIMK-1 and LIMK-2 suggest distinct roles during development, with LIMK-1 being implicated in inhibiting neuronal differentiation in certain contexts. Understanding LIMK-1 function and regulation is essential for elucidating contributions to cellular processes and potential therapeutic targets in diseases characterized by aberrant cell motility.

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LIMK-1 Antibody (C-10) References:

1. Head, neck, and spines: a role for LIMK-1 in the hippocampus.  |  Sarmiere, PD. and Bamburg, JR. 2002. Neuron. 35: 3-5. PMID: 12123600
2. Abnormal spine morphology and enhanced LTP in LIMK-1 knockout mice.  |  Meng, Y., et al. 2002. Neuron. 35: 121-33. PMID: 12123613
3. miR-143-3p targeting LIM domain kinase 1 suppresses the progression of triple-negative breast cancer cells.  |  Li, D., et al. 2017. Am J Transl Res. 9: 2276-2285. PMID: 28559978
4. MiR-519d-3p suppresses breast cancer cell growth and motility via targeting LIM domain kinase 1.  |  Li, D., et al. 2018. Mol Cell Biochem. 444: 169-178. PMID: 29188531
5. 6-Hydroxythiobinupharidine Inhibits Migration of LM8 Osteosarcoma Cells by Decreasing Expression of LIM Domain Kinase 1.  |  Yoshizawa, M., et al. 2019. Anticancer Res. 39: 6507-6513. PMID: 31810915
6. HUNK inhibits epithelial-mesenchymal transition of CRC via direct phosphorylation of GEF-H1 and activating RhoA/LIMK-1/CFL-1.  |  Han, X., et al. 2023. Cell Death Dis. 14: 327. PMID: 37193711
7. LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family.  |  Nunoue, K., et al. 1995. Oncogene. 11: 701-10. PMID: 7651734
8. Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs.  |  Okano, I., et al. 1995. J Biol Chem. 270: 31321-30. PMID: 8537403
9. Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the developing rat.  |  Mori, T., et al. 1997. Brain Res Mol Brain Res. 45: 247-54. PMID: 9149099
10. Inhibition of activated Ras-induced neuronal differentiation of PC12 cells by the LIM domain of LIM-kinase 1.  |  Higuchi, O., et al. 1997. Oncogene. 14: 1819-25. PMID: 9150388
11. Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.  |  Yang, N., et al. 1998. Nature. 393: 809-12. PMID: 9655398
12. A novel transcript encoding truncated LIM kinase 2 is specifically expressed in male germ cells undergoing meiosis.  |  Takahashi, H., et al. 1998. Biochem Biophys Res Commun. 249: 138-45. PMID: 9705845

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIMK-1 Antibody (C-10) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-LIMK-1 Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIMK-1 Antibody (C-10) Specifications

Alternate Product Name: LIMK1

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 136-219

Epitope Target: LIMK-1

Epitope Species: human

Product Reactivity/Detection: LIMK-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for LIMK-1 Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin