LGR4 Antibody (C-12): sc-390630

LGR4 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects LGR4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LGR4 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4 or GPR48, is a 951 amino acid multi-pass membrane protein characterized by 15 leucine-rich repeats, which are crucial for structural integrity and function as a receptor. LGR4 is predominantly expressed in several tissues, including testis, ovary, placenta, stomach, heart, kidney, pancreas, and spleen, where LGR4 plays a significant role as an orphan receptor, potentially influencing various physiological processes. LGR4 is implicated in cancer biology, as LGR4 is overexpressed in multiple cancer types, contributing to enhanced invasiveness and metastasis of carcinoma cells, thereby highlighting LGR4′s importance in tumor progression and making LGR4 a potential target for therapeutic intervention.

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LGR4 Antibody (C-12) References:

1. Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping.  |  Loh, ED., et al. 2000. Cytogenet Cell Genet. 89: 2-5. PMID: 10894923
2. Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor.  |  Loh, ED., et al. 2001. Biochem Biophys Res Commun. 282: 757-64. PMID: 11401528
3. Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis.  |  Gao, Y., et al. 2006. Cancer Res. 66: 11623-31. PMID: 17178856
4. Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2.  |  Weng, J., et al. 2008. Proc Natl Acad Sci U S A. 105: 6081-6. PMID: 18424556
5. Impaired hair placode formation with reduced expression of hair follicle-related genes in mice lacking Lgr4.  |  Mohri, Y., et al. 2008. Dev Dyn. 237: 2235-42. PMID: 18651655
6. Inactivation of G-protein-coupled receptor 48 (Gpr48/Lgr4) impairs definitive erythropoiesis at midgestation through down-regulation of the ATF4 signaling pathway.  |  Song, H., et al. 2008. J Biol Chem. 283: 36687-97. PMID: 18955481
7. Lgr4 Governs a Pro-Inflammatory Program in Macrophages to Antagonize Post-Infarction Cardiac Repair.  |  Huang, CK., et al. 2020. Circ Res. 127: 953-973. PMID: 32600176
8. RSPO2 and RANKL signal through LGR4 to regulate osteoclastic premetastatic niche formation and bone metastasis.  |  Yue, Z., et al. 2022. J Clin Invest. 132: PMID: 34847079
9. Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit Vascular Calcification.  |  Chen, Y., et al. 2022. Circ Res. 131: 1037-1054. PMID: 36354004
10. Targeted activation of ferroptosis in colorectal cancer via LGR4 targeting overcomes acquired drug resistance.  |  Zheng, H., et al. 2024. Nat Cancer. 5: 572-589. PMID: 38291304
11. Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice.  |  Liang, Y., et al. 2024. Nat Commun. 15: 4393. PMID: 38782937
12. Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region.  |  Hsu, SY., et al. 1998. Mol Endocrinol. 12: 1830-45. PMID: 9849958

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LGR4 Antibody (C-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-LGR4 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LGR4 Antibody (C-12) Specifications

Alternate Product Name: LGR4

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 810-951

Epitope Target: LGR4

Epitope Species: human

Product Reactivity/Detection: LGR4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for LGR4 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin