Lex A Antibody (E-7): sc-365999
- Lex A Antibody (E-7) is a mouse monoclonal IgG2b κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of Lex A of E. coli origin
- recommended for detection of Lex A and Lex A fusion proteins by WB, IP, IF and ELISA
- See Lex A (C-11): sc-390386 for Lex A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Lex A Antibody (E-7) for WB applications. These reagents are now offered in bundles with Lex A Antibody (E-7) (see ordering information below).
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Lex A Antibody (E-7) is a mouse monoclonal IgG2b antibody that detects Lex A by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Lex A is a transcription factor that plays a crucial role in regulating gene expression in response to environmental signals, making Lex A essential for cellular adaptation and survival. Lex A (E-7) antibody primarily detects nuclear localization, where Lex A binds to specific DNA sequences to modulate target gene transcription. Nuclear localization allows Lex A to directly influence gene expression involved in various cellular processes, including stress response and metabolic regulation. Furthermore, Lex A interacts with several other proteins, such as transcriptional coactivator CBP, which enhances Lex A′s ability to activate gene expression. Anti-Lex A antibody (E-7) serves as an invaluable tool for researchers studying gene regulation and protein interactions, providing insights into complex networks governing cellular function and response to stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Lex A Antibody (E-7) References:
- Mechanism of specific LexA cleavage: autodigestion and the role of RecA coprotease. | Little, JW. 1991. Biochimie. 73: 411-21. PMID: 1911941
- DNA binding properties of the LexA repressor. | Schnarr, M., et al. 1991. Biochimie. 73: 423-31. PMID: 1911942
- The LexA regulated genes of the Clostridium difficile. | Walter, BM., et al. 2014. BMC Microbiol. 14: 88. PMID: 24713082
- Specificity determinants for autoproteolysis of LexA, a key regulator of bacterial SOS mutagenesis. | Mo, CY., et al. 2014. Biochemistry. 53: 3158-68. PMID: 24779472
- An SOS Regulon under Control of a Noncanonical LexA-Binding Motif in the Betaproteobacteria. | Sanchez-Alberola, N., et al. 2015. J Bacteriol. 197: 2622-30. PMID: 25986903
- A corepressor participates in LexA-independent regulation of error-prone polymerases in Acinetobacter. | Peterson, MA., et al. 2020. Microbiology (Reading). 166: 212-226. PMID: 31687925
- Two components of DNA replication-dependent LexA cleavage. | Myka, KK. and Marians, KJ. 2020. J Biol Chem. 295: 10368-10379. PMID: 32513870
- Integration of molecular modelling and in vitro studies to inhibit LexA proteolysis. | Schuurs, ZP., et al. 2023. Front Cell Infect Microbiol. 13: 1051602. PMID: 36936756
- The LexA-RecA* structure reveals a cryptic lock-and-key mechanism for SOS activation. | Cory, MB., et al. 2024. Nat Struct Mol Biol. 31: 1522-1531. PMID: 38755298
- A LexA mutant repressor with a relaxed inter-domain linker. | Oertel-Buchheit, P., et al. 1998. Protein Sci. 7: 512-5. PMID: 9521130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lex A Antibody (E-7) | sc-365999 | 200 µg/ml | $322.00 | |||
Lex A Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-535294 | 200 µg Ab; 40 µg BP | $361.00 | |||
Lex A Antibody (E-7): m-IgG2b BP-HRP Bundle | sc-549384 | 200 µg Ab; 10 µg BP | $361.00 | |||
Lex A (E-7) Neutralizing Peptide | sc-365999 P | 100 µg/0.5 ml | $69.00 |