LEF-1 Antibody (B-10): sc-374412

LEF-1 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects LEF-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LEF-1 Antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LEF-1 plays a crucial role in the Wnt signaling pathway, which regulates gene expression during development and cellular differentiation. LEF-1 is involved in transcriptional regulation of target genes critical for lymphoid cell development and other processes. LEF-1 function is particularly important because aberrations in the Wnt signaling pathway can lead to various diseases, including cancer. LEF-1 interaction with β-catenin in the nucleus enhances expression of Wnt target genes, while mutations in the adenomatous polyposis coli (APC) gene can disrupt this regulatory mechanism, resulting in uncontrolled cell proliferation. Understanding LEF-1 and its regulatory pathways is vital for developing therapeutic strategies against cancers associated with Wnt signaling dysregulation.

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LEF-1 Antibody (B-10) References:

1. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells.  |  Staal, FJ., et al. 1999. Int Immunol. 11: 317-23. PMID: 10221643
2. The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization.  |  van de Wetering, M., et al. 1992. J Biol Chem. 267: 8530-6. PMID: 1569101
3. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box.  |  van de Wetering, M., et al. 1991. EMBO J. 10: 123-32. PMID: 1989880
4. Sox2 modulates Lef-1 expression during airway submucosal gland development.  |  Xie, W., et al. 2014. Am J Physiol Lung Cell Mol Physiol. 306: L645-60. PMID: 24487391
5. Downregulation of Lymphoid enhancer-binding factor 1 (LEF-1) expression (by immunohistochemistry and/ flow cytometry) in chronic Lymphocytic Leukemia with atypical immunophenotypic and cytologic features.  |  Soliman, DS., et al. 2021. Int J Lab Hematol. 43: 515-525. PMID: 33314668
6. The β-catenin-LINC00183-miR-371b-5p-Smad2/LEF1 axis promotes adult T-cell lymphoblastic lymphoma progression and chemoresistance.  |  Huang, WJ., et al. 2023. J Exp Clin Cancer Res. 42: 105. PMID: 37106379
7. Targeting LEF1-mediated epithelial-mesenchymal transition reverses lenvatinib resistance in hepatocellular carcinoma.  |  Li, X., et al. 2024. Invest New Drugs. 42: 185-195. PMID: 38372948
8. An HMG-box-containing T-cell factor required for thymocyte differentiation.  |  Verbeek, S., et al. 1995. Nature. 374: 70-4. PMID: 7870176
9. Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.  |  Morin, PJ., et al. 1997. Science. 275: 1787-90. PMID: 9065402
10. Wnt-1 induces growth, cytosolic beta-catenin, and Tcf/Lef transcriptional activation in Rat-1 fibroblasts.  |  Young, CS., et al. 1998. Mol Cell Biol. 18: 2474-85. PMID: 9566868
11. Control of neural crest cell fate by the Wnt signalling pathway.  |  Dorsky, RI., et al. 1998. Nature. 396: 370-3. PMID: 9845073
12. Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium.  |  Barker, N., et al. 1999. Am J Pathol. 154: 29-35. PMID: 9916915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LEF-1 Antibody (B-10) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-LEF-1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LEF-1 Antibody (B-10) Specifications

Alternate Product Name: LEF1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 61-130

Epitope Target: LEF-1

Epitope Species: human

Product Reactivity/Detection: LEF-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for LEF-1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent