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LDH-D Antibody (G-8): sc-374127

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Datasheets
  • LDH-D Antibody (G-8) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-195 mapping at the N-terminus of LDH-D of human origin
  • recommended for detection of LDH-D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LDH-D Antibody (G-8) for WB and IHC(P) applications. These reagents are now offered in bundles with LDH-D Antibody (G-8) (see ordering information below).
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    LDH-D Antibody (G-8) is a mouse monoclonal IgG2b antibody that detects lactate dehydrogenase D (LDH-D) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LDH-D plays a crucial role in cellular metabolism, specifically in the conversion of D-lactate to pyruvate, which is essential for energy production in cells. LDH-D (G-8) antibody targets a protein predominantly located in the mitochondria, where LDH-D participates in the metabolic pathways that generate ATP, the energy currency of the cell. The mitochondrial localization of LDH-D is significant because LDH-D allows for efficient energy production in tissues with high metabolic demands, such as the liver and heart. Additionally, LDH-D exists in two alternatively spliced isoforms, which may have distinct regulatory roles in metabolic processes. LDH-D′s ability to interact with other metabolic enzymes further underscores LDH-D′s importance in maintaining cellular energy homeostasis and responding to metabolic stress. With specific reactivity and versatile applications, anti-LDH-D antibody (G-8) serves as an invaluable tool for researchers studying metabolic pathways and energy regulation in various biological contexts.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

    Download thiomersal SDS

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    LDH-D Antibody (G-8)

    sc-374127
    200 µg/ml
    $322.00

    LDH-D Antibody (G-8): m-IgGκ BP-HRP Bundle

    sc-535393
    200 µg Ab; 40 µg BP
    $361.00

    LDH-D Antibody (G-8): m-IgG2b BP-HRP Bundle

    sc-549406
    200 µg Ab; 10 µg BP
    $361.00

    Can LDH-D (G-8): sc-374127 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: cjMara
    Thank you for your question. Yes, LDH-D (G-8): sc-374127 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2026, m_11, d_4, h_4CST
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    Rated 5 out of 5 by MS from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
    Date published: 2015-05-02
    Rated 5 out of 5 by FL from Robust signal in WB in mouse transfectedRobust signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-01-03
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    LDH-D Antibody (G-8) is rated 5.0 out of 5 by 2.
    • y_2026, m_11, d_5, h_5
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