LDH-B Antibody (431.1): sc-100775

LDH-B Antibody (431.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the LDH-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-LDH-B antibody (431.1) is available as the non-conjugated format. The lactate dehydrogenase B (LDH-B) protein plays a crucial role in cellular metabolism, specifically in the conversion of L-lactate to pyruvate, which is essential for energy production during anaerobic conditions. This enzymatic activity is vital for tissues that rely on anaerobic glycolysis, such as cardiac muscle, where LDH-B is predominantly expressed. Proper functioning of LDH-B is important not only for energy metabolism but also for maintaining the balance of lactate and pyruvate in the body, which can influence various physiological processes and metabolic pathways. Alterations in LDH-B levels have been associated with various diseases, including heart conditions and certain cancers, making LDH-B a significant biomarker for clinical diagnostics. The lactate dehydrogenase family consists of three members—LDH-A, LDH-B, and LDH-C—each exhibiting distinct tissue distribution patterns, with LDH-B being particularly important in the heart. The genes encoding human LDH-A and LDH-C are located on chromosome 11, while the human LDH-B gene is mapped to chromosome 12, highlighting the genetic basis for its expression and function.

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LDH-B Antibody (431.1) References:

1. [The role of tumor markers in the treatment of germ cell tumor].  |  Niwakawa, M. and Tobisu, K. 2001. Gan To Kagaku Ryoho. 28: 1159-65. PMID: 11525037
2. Molecular characterization of genetic mutations in human lactate dehydrogenase (LDH) B (H) variant.  |  Sudo, K., et al. 1992. Hum Genet. 89: 158-62. PMID: 1587525
3. Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli.  |  LeVan, KM. and Goldberg, E. 1991. Biochem J. 273 (Pt 3): 587-92. PMID: 1996957
4. Locus determining the human sperm-specific lactate dehydrogenase, LDHC, is syntenic with LDHA.  |  Edwards, YH., et al. 1987. Dev Genet. 8: 219-32. PMID: 2844458
5. Human testicular lactate dehydrogenase-C gene is interrupted by six introns at positions homologous to those of LDH-A (muscle) and LDH-B (heart) genes.  |  Takano, T. and Li, SS. 1989. Biochem Biophys Res Commun. 159: 579-83. PMID: 2930531
6. Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment.  |  Frank, AC., et al. 2021. Theranostics. 11: 7570-7588. PMID: 34158867
7. Integrated analysis of single cell-RNA sequencing and Mendelian randomization identifies lactate dehydrogenase B as a target of melatonin in ischemic stroke.  |  Shi, F., et al. 2024. CNS Neurosci Ther. 30: e14741. PMID: 38702940
8. Lactate dehydrogenase B noncanonically promotes ferroptosis defense in KRAS-driven lung cancer.  |  Zhao, L., et al. 2024. Cell Death Differ.. PMID: 39643712
9. Manipulation of anti-LDH-B response by T suppressor factors.  |  Ikezawa, Z., et al. 1984. J Immunol. 132: 1605-7. PMID: 6607942
10. Lactate dehydrogenase M-subunit deficiencies: clinical features, metabolic background, and genetic heterogeneities.  |  Kanno, T. and Maekawa, M. 1995. Muscle Nerve Suppl. 3: S54-60. PMID: 7603529
11. Methods for the separation of lactate dehydrogenases and clinical significance of the enzyme.  |  Kopperschläger, G. and Kirchberger, J. 1996. J Chromatogr B Biomed Appl. 684: 25-49. PMID: 8906464
12. Lactate dehydrogenase from the hyperthermophilic bacterium thermotoga maritima: the crystal structure at 2.1 A resolution reveals strategies for intrinsic protein stabilization.  |  Auerbach, G., et al. 1998. Structure. 6: 769-81. PMID: 9655830

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LDH-B Antibody (431.1) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-LDH-B Antibody (431.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LDH-B Antibody (431.1) Specifications

Alternate Product Name: LDHB

Product Clone Name: 431.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-335

Epitope Target: LDH-B

Epitope Species: human

Product Reactivity/Detection: LDH-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for LDH-B Antibody (431.1): N/A