LDH Antibody (H-10): sc-133123

LDH Antibody (H-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects lactate dehydrogenase (LDH) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LDH (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The lactate dehydrogenase family, including LDH-A, LDH-B, and LDH-C, plays a crucial role in cellular metabolism by catalyzing the conversion of L-lactate and NAD to pyruvate and NADH, marking the final step of anaerobic glycolysis. This enzymatic activity is vital for energy production, especially in tissues that rely heavily on anaerobic metabolism, such as muscle during intense exercise. Each LDH isoform exhibits distinct tissue distribution, with LDH-A predominantly found in muscle, LDH-B in the heart, and LDH-C restricted to the testis and sperm, making these isoforms important markers for various physiological and pathological conditions, including germ cell tumors. The genes encoding human LDH-A and LDH-C are located on chromosome 11, while the LDH-B gene is found on chromosome 12, and deficiencies in the LDH-A gene have been associated with exertional myoglobinuria, highlighting the clinical significance of LDH in metabolic disorders.

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LDH Antibody (H-10) References:

1. [The role of tumor markers in the treatment of germ cell tumor].  |  Niwakawa, M. and Tobisu, K. 2001. Gan To Kagaku Ryoho. 28: 1159-65. PMID: 11525037
2. Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli.  |  LeVan, KM. and Goldberg, E. 1991. Biochem J. 273 (Pt 3): 587-92. PMID: 1996957
3. Locus determining the human sperm-specific lactate dehydrogenase, LDHC, is syntenic with LDHA.  |  Edwards, YH., et al. 1987. Dev Genet. 8: 219-32. PMID: 2844458
4. LDH-A negatively regulates dMMR in colorectal cancer.  |  Zhang, Y., et al. 2021. Cancer Sci. 112: 3050-3063. PMID: 34110068
5. The multiple roles of LDH in cancer.  |  Claps, G., et al. 2022. Nat Rev Clin Oncol. 19: 749-762. PMID: 36207413
6. Rationale for LDH-targeted cancer immunotherapy.  |  Miholjcic, TBS., et al. 2023. Eur J Cancer. 181: 166-178. PMID: 36657325
7. The lactate dehydrogenase (LDH) isoenzyme spectrum enables optimally controlling T cell glycolysis and differentiation.  |  Chen, X., et al. 2023. Sci Adv. 9: eadd9554. PMID: 36961904
8. LDH-Based 'Smart' Films for Corrosion Sensing and Protection.  |  Zhao, X., et al. 2023. Materials (Basel). 16: PMID: 37176365
9. LDH and melanoma.  |  Finck, SJ., et al. 1983. Cancer. 51: 840-3. PMID: 6821850
10. Lactate dehydrogenase M-subunit deficiencies: clinical features, metabolic background, and genetic heterogeneities.  |  Kanno, T. and Maekawa, M. 1995. Muscle Nerve Suppl. 3: S54-60. PMID: 7603529
11. Methods for the separation of lactate dehydrogenases and clinical significance of the enzyme.  |  Kopperschläger, G. and Kirchberger, J. 1996. J Chromatogr B Biomed Appl. 684: 25-49. PMID: 8906464
12. Lactate dehydrogenase from the hyperthermophilic bacterium thermotoga maritima: the crystal structure at 2.1 A resolution reveals strategies for intrinsic protein stabilization.  |  Auerbach, G., et al. 1998. Structure. 6: 769-81. PMID: 9655830

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LDH Antibody (H-10) has 59 citations in a variety of scientific publications.

Primary antibodies like Anti-LDH Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LDH Antibody (H-10) Specifications

Alternate Product Name: LDH

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 173-332

Epitope Target: LDH-A

Epitope Species: human

Product Reactivity/Detection: LDH-A, LDH-B, LDH-C, LDH-A-like 6A and LDH-A-like 6B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 59 citations in various scientific publications

Available Conjugates/Forms for LDH Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin