Lck Antibody (3A5): sc-433

Lck Antibody (3A5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Lck protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Lck (3A5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lck protein, a member of the Src family of tyrosine kinases, plays a crucial role in T cell signaling, particularly in the activation of T cell antigen receptors (TCRs). This activation is vital for the immune response, as it triggers downstream signaling pathways that lead to T cell proliferation, differentiation, and cytokine production. Lck protein is primarily localized in the plasma membrane of T cells, where it associates with the TCR complex, ensuring that it is positioned to effectively transmit signals upon receptor engagement. The human Lck gene is located on chromosome 1p35.1 and encodes a 509 amino acid protein, which is essential for the regulation of immune responses. By understanding the function and localization of Lck, researchers can better appreciate its significance in T cell biology and its potential implications in immunological disorders.

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Lck Antibody (3A5) References:

1. Differential T-cell antigen receptor signaling mediated by the Src family kinases Lck and Fyn.  |  Denny, MF., et al. 2000. Mol Cell Biol. 20: 1426-35. PMID: 10648627
2. Kinases of the Src family: structure and functions.  |  Tatosyan, AG. and Mizenina, OA. 2000. Biochemistry (Mosc). 65: 49-58. PMID: 10702640
3. Src kinase-mediated signaling in leukocytes.  |  Korade-Mirnics, Z. and Corey, SJ. 2000. J Leukoc Biol. 68: 603-13. PMID: 11073097
4. Selected glimpses into the activation and function of Src kinase.  |  Bjorge, JD., et al. 2000. Oncogene. 19: 5620-35. PMID: 11114743
5. SRC family kinases mediate epithelial Na+ channel inhibition by endothelin.  |  Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
6. Asparagine enhances LCK signalling to potentiate CD8+ T-cell activation and anti-tumour responses.  |  Wu, J., et al. 2021. Nat Cell Biol. 23: 75-86. PMID: 33420490
7. LAG3 associates with TCR-CD3 complexes and suppresses signaling by driving co-receptor-Lck dissociation.  |  Guy, C., et al. 2022. Nat Immunol. 23: 757-767. PMID: 35437325
8. LCK inhibition downregulates YAP activity and is therapeutic in patient-derived models of cholangiocarcinoma.  |  Conboy, CB., et al. 2023. J Hepatol. 78: 142-152. PMID: 36162702
9. CD28-CAR-T cell activation through FYN kinase signaling rather than LCK enhances therapeutic performance.  |  Wu, L., et al. 2023. Cell Rep Med. 4: 100917. PMID: 36696897
10. A partial human LCK defect causes a T cell immunodeficiency with intestinal inflammation.  |  Lui, VG., et al. 2024. J Exp Med. 221: PMID: 37962568
11. Genetic organization of human proto-oncogenes.  |  Sakaguchi, AY. 1983. Prog Clin Biol Res. 119: 93-103. PMID: 6191340
12. Insights into Src kinase functions: structural comparisons.  |  Williams, JC., et al. 1998. Trends Biochem Sci. 23: 179-84. PMID: 9612082

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lck Antibody (3A5) has 313 citations in a variety of scientific publications.

Primary antibodies like Anti-Lck Antibody (3A5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Lck Antibody (3A5) Specifications

Alternate Product Name: p56LCK

Product Clone Name: 3A5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-225

Epitope Target: Lck

Epitope Species: mouse

Product Reactivity/Detection: Lck p56

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 313 citations in various scientific publications

Available Conjugates/Forms for Lck Antibody (3A5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin