Latrophilin-1 Antibody (A-4): sc-514562
- Latrophilin-1 Antibody (A-4) is a mouse monoclonal IgG1 κ Latrophilin-1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 335-357 within an N-terminal extracellular domain of Latrophilin-1 of human origin
- Latrophilin-1 Antibody (A-4) is recommended for detection of Latrophilin-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Latrophilin-1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Latrophilin-1 Antibody (A-4) for WB applications. These reagents are now offered in bundles with Latrophilin-1 Antibody (A-4) (see ordering information below).
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Latrophilin-1 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Latrophilin-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Latrophilin-1 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Latrophilin-1 plays a crucial role in synaptic transmission as a receptor for α-latrotoxin, a potent neurotoxin derived from black widow spider venom. Upon binding with α-latrotoxin, anti-Latrophilin-1 antibody (A-4) initiates synaptic vesicle exocytosis through both calcium-dependent and independent pathways, leading to synaptic vesicle depletion, which is vital for neurotransmitter release and neuronal communication. Latrophilin-1 is predominantly expressed in brain and endocrine cells, highlighting its importance in neurobiology and endocrine signaling. The Latrophilin family, including Latrophilin-2 and Latrophilin-3, exhibits distinct tissue distribution and functions, with Latrophilin-1 being the most extensively studied member, underscoring its significance in both physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Latrophilin-1 Antibody (A-4) References:
- The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. | Matsushita, H., et al. 1999. FEBS Lett. 443: 348-52. PMID: 10025961
- Alpha-latrotoxin and its receptors CIRL (latrophilin) and neurexin 1 alpha mediate effects on secretion through multiple mechanisms. | Bittner, MA. 2000. Biochimie. 82: 447-52. PMID: 10865131
- alpha-latrotoxin forms calcium-permeable membrane pores via interactions with latrophilin or neurexin. | Van Renterghem, C., et al. 2000. Eur J Neurosci. 12: 3953-62. PMID: 11069591
- alpha-Latrotoxin and its receptors: neurexins and CIRL/latrophilins. | Südhof, TC. 2001. Annu Rev Neurosci. 24: 933-62. PMID: 11520923
- Spiders of medical importance in the Asia-Pacific: atracotoxin, latrotoxin and related spider neurotoxins. | Nicholson, GM. and Graudins, A. 2002. Clin Exp Pharmacol Physiol. 29: 785-94. PMID: 12165044
- The multiple actions of black widow spider toxins and their selective use in neurosecretion studies. | Ushkaryov, YA., et al. 2004. Toxicon. 43: 527-42. PMID: 15066411
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Latrophilin-1 Antibody (A-4) | sc-514562 | 200 µg/ml | $322.00 | |||
Latrophilin-1 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-531159 | 200 µg Ab; 10 µg BP | $361.00 | |||
Latrophilin-1 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524769 | 200 µg Ab, 40 µg BP | $361.00 | |||
Latrophilin-1 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-544419 | 200 µg Ab; 20 µg BP | $361.00 | |||
Latrophilin-1 Antibody (A-4) AC | sc-514562 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Latrophilin-1 Antibody (A-4) HRP | sc-514562 HRP | 200 µg/ml | $322.00 | |||
Latrophilin-1 Antibody (A-4) FITC | sc-514562 FITC | 200 µg/ml | $336.00 | |||
Latrophilin-1 Antibody (A-4) PE | sc-514562 PE | 200 µg/ml | $349.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 488 | sc-514562 AF488 | 200 µg/ml | $364.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 546 | sc-514562 AF546 | 200 µg/ml | $364.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 594 | sc-514562 AF594 | 200 µg/ml | $364.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 647 | sc-514562 AF647 | 200 µg/ml | $364.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 680 | sc-514562 AF680 | 200 µg/ml | $364.00 | |||
Latrophilin-1 Antibody (A-4) Alexa Fluor® 790 | sc-514562 AF790 | 200 µg/ml | $364.00 | |||
Latrophilin-1 (A-4) Neutralizing Peptide | sc-514562 P | 100 µg/0.5 ml | $69.00 |