Date published: 2025-9-5
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LASS2 Antibody (C-11) - Western Blotting - Image 289632
LASS2 抗体 (C-11): sc-390745. HeLa (A), Hep G2 (B), Jurkat (C), K-562 (D) 和 Ramos (E) 全细胞裂解液中 LASS2 表达的 Western 印迹分析.
LASS2 抗体 (C-11): sc-390745. HeLa (A), Hep G2 (B), Jurkat (C), K-562 (D) 和 Ramos (E) 全细胞裂解液中 LASS2 表达的 Western 印迹分析.

LASS2 抗体 (C-11): sc-390745

5.0(7)
寫評論提問

说明书
  • LASS2 抗体 C-11 是小鼠单克隆 IgG1 κ，LASS2抗体, 在6篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列141-180位于human物种的LASS2的内部区域内
  • LASS2 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 LASS2; 也和以下物种反应，包括： 和 equine and canine
  • 抗LASS2抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LASS2 (C-11): sc-390745的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成LASS2 抗体 (C-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 LASS 抗体 包括 LASS1, LASS2, LASS4和LASS6

LASS2抗体（C-11）是一种IgG1 κ小鼠单克隆LASS2抗体（也称为LASS2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的LASS2蛋白。LASS2抗体（C-11）既有非偶联抗LASS2抗体形式，也有多种偶联形式的抗LASS2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。从酵母到哺乳动物，LASS（长寿保证同源物）家族成员都高度保守。已经鉴定出参与鞘脂合成的这个蛋白质家族的六个成员（LASS1、LASS2、LASS3、LASS4、LASS5和LASS6）。LASS2是一种380个氨基酸的多通道膜蛋白，在肾、肝、脑、心、胎盘和肺中表达。LASS2抑制癌细胞的生长，并参与鞘脂的合成。LASS2的过量产生会增加长链神经酰胺的水平，如C22:0-和C24:0-神经酰胺。N端天冬酰胺残基作为内质网膜腔侧糖基化的位点。LASS2与几种膜相关受体或转运体相互作用，包括ASGPR1、ASGPR2和OCT1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LASS2 抗体 (C-11) 参考文献:

  1. 人类膀胱癌中新的肿瘤转移抑制基因LASS2的表达和预后意义。  |  Wang, H., et al. 2012. Med Oncol. 29: 1921-7. PMID: 21755371
  2. LASS2通过抑制V-ATP酶质子泵的活性，抵消酸性肿瘤微环境的影响，从而增强乳腺癌的化疗敏感性。  |  Fan, S., et al. 2013. Oncogene. 32: 1682-90. PMID: 22580606
  3. 新型肿瘤抑制基因LASS2/TMSG1通过其同源结构域与液泡ATP酶相互作用。  |  Yu, W., et al. 2013. J Cell Biochem. 114: 570-83. PMID: 22991218
  4. 肝癌抑制基因Lass2的敲除可下调miR-694的表达水平。  |  Lu, X., et al. 2014. Oncol Rep. 32: 2696-702. PMID: 25333455
  5. LASS2/TMSG1通过调节液泡ATP酶活性抑制乳腺癌细胞在体外生长和侵袭。  |  Mei, F., et al. 2015. Tumour Biol. 36: 2831-44. PMID: 25501280
  6. miR-9通过靶向膀胱癌中的LASS2促进细胞增殖并抑制细胞凋亡。  |  Wang, H., et al. 2015. Tumour Biol. 36: 9631-40. PMID: 26150338
  7. 唾液酸蛋白受体通过LASS2介导的V-ATP酶活性抑制，抑制肝癌的转移。  |  Gu, D., et al. 2016. Cancer Lett. 379: 107-16. PMID: 27241665
  8. LASS2通过影响线粒体动力学、细胞周期和核因子-κB通路，抑制HepG2细胞的增殖并诱导其凋亡。  |  Yang, Y., et al. 2019. Oncol Rep. 41: 3005-3014. PMID: 30896811
  9. miR-3622a通过下调LASS2促进膀胱癌细胞的增殖和侵袭。  |  Fu, S., et al. 2019. Gene. 701: 23-31. PMID: 30898713
  10. Hsa-miR-221-3p通过靶向LASS2和MBD2促进HER2阳性乳腺癌细胞的增殖和迁移。  |  Shao, X., et al. 2022. Histol Histopathol. 37: 1099-1112. PMID: 35734966

订购信息

产品名称产品编号规格价格数量收藏夹

LASS2 抗体 (C-11)

sc-390745
200 µg/ml
$316.00

LASS2 抗体 (C-11) AC

sc-390745 AC
500 µg/ml, 25% agarose
$416.00

LASS2 抗体 (C-11) HRP

sc-390745 HRP
200 µg/ml
$316.00

LASS2 抗体 (C-11) FITC

sc-390745 FITC
200 µg/ml
$330.00

LASS2 抗体 (C-11) PE

sc-390745 PE
200 µg/ml
$343.00

LASS2 抗体 (C-11) Alexa Fluor® 488

sc-390745 AF488
200 µg/ml
$357.00

LASS2 抗体 (C-11) Alexa Fluor® 546

sc-390745 AF546
200 µg/ml
$357.00

LASS2 抗体 (C-11) Alexa Fluor® 594

sc-390745 AF594
200 µg/ml
$357.00

LASS2 抗体 (C-11) Alexa Fluor® 647

sc-390745 AF647
200 µg/ml
$357.00

LASS2 抗体 (C-11) Alexa Fluor® 680

sc-390745 AF680
200 µg/ml
$357.00

LASS2 抗体 (C-11) Alexa Fluor® 790

sc-390745 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with LASS2 (C-11): sc-390745 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Superwe used this antibody in western blot in 1:300 dilution for 2 hours at room temperature and it worked very well. Cell extracts derived from BC3 cells.
Date published: 2024-11-14
Rated 5 out of 5 by from Both good result for IF and WBWorks very well for Western Blot analysis and Immunofluorescence. For Western blot 1:1000 dilution is used. An specific band is at 45kDa . For IF we got clear signals using a 1:100 antibody dilution. Specifcity was confirmed by IF staining of Hela cell.
Date published: 2017-08-12
Rated 5 out of 5 by from great antibodyI tried this antibody with mouse RAW 264.7 cells, the western blot result shown a clean and strong band.
Date published: 2017-07-20
Rated 5 out of 5 by from Great productThe LASS2 Antibody (C-11): sc-390745 works very well for Western blot applications, showing clear banding with minimal background noise.
Date published: 2017-02-07
Rated 5 out of 5 by from Ramos whole cell lysatesThis antibody works great for western blot in Ramos whole cell lysates, at a dilution of 1:1000 in 5% nonfat milk in got strong signal.
Date published: 2017-01-15
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Great Western Blot data of LASS2 expressionGreat Western Blot data of LASS2 expression in HeLa, Hep G2, Jurkat, K-562 and Ramos whole cell lysates. -SCBT QC
Date published: 2014-09-02
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LASS2 抗体 (C-11) is rated 5.0 out of 5 by 7.
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