Laminin β-2 Antibody (C4): sc-59980

Laminin β-2 Antibody (C4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Laminin beta-2 protein of mouse, rat, human, bovine, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Laminin beta-2 antibody (C4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminin beta-2 is a critical component of the extracellular matrix, particularly in the basal lamina, where Laminin beta-2 contributes to the structural integrity and function of tissues. A unique structure consisting of three polypeptide chains (α, β, and γ) allows Laminin beta-2 to form a network that supports cell adhesion, migration, and differentiation. This structural role proves vital during embryonic development, facilitating cell migration along basement membranes and ensuring proper tissue organization and function. Additionally, Laminin beta-2 has been implicated in cancer biology, particularly in melanoma, where Laminin beta-2 promotes tumor cell migration along the abluminal surface of blood vessels, a process known as extra-vascular migratory metastasis. Such involvement in both normal physiological processes and pathological conditions makes Laminin beta-2 monoclonal antibody (C4) an essential tool for researchers studying cell behavior in development and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Laminin β-2 Antibody (C4) References:

1. Expression of laminin beta2 chain in normal human bone marrow.  |  Vogel, W., et al. 1999. Blood. 94: 1143-5. PMID: 10454804
2. Disruption of laminin beta2 chain production causes alterations in morphology and function in the CNS.  |  Libby, RT., et al. 1999. J Neurosci. 19: 9399-411. PMID: 10531444
3. Mutations in the human laminin beta2 (LAMB2) gene and the associated phenotypic spectrum.  |  Matejas, V., et al. 2010. Hum Mutat. 31: 992-1002. PMID: 20556798
4. Laminin β2 Chain Regulates Cell Cycle Dynamics in the Developing Retina.  |  Serjanov, D., et al. 2021. Front Cell Dev Biol. 9: 802593. PMID: 35096830
5. Heterozygous laminin β2 mutation in C3 glomerulopathy.  |  Balwani, MR., et al. 2021. Saudi J Kidney Dis Transpl. 32: 1479-1481. PMID: 35532722
6. Basal lamina assembly.  |  Yurchenco, PD. and O'Rear, JJ. 1994. Curr Opin Cell Biol. 6: 674-81. PMID: 7833047
7. Laminins: a family of diverse multifunctional molecules of basement membranes.  |  Aumailley, M. and Krieg, T. 1996. J Invest Dermatol. 106: 209-214. PMID: 8601717
8. Mechanism of laminin chain assembly into a triple-stranded coiled-coil structure.  |  Nomizu, M., et al. 1996. Biochemistry. 35: 2885-93. PMID: 8608125
9. Laminin interactions important for basement membrane assembly are promoted by zinc and implicate laminin zinc finger-like sequences.  |  Ancsin, JB. and Kisilevsky, R. 1996. J Biol Chem. 271: 6845-51. PMID: 8636109
10. Structure and function of basement membranes.  |  Engvall, E. 1995. Int J Dev Biol. 39: 781-7. PMID: 8645562
11. Structural organization of the human and mouse laminin beta2 chain genes, and alternative splicing at the 5' end of the human transcript.  |  Durkin, ME., et al. 1996. J Biol Chem. 271: 13407-16. PMID: 8662701
12. Laminin-binding integrins in tumor progression and metastasis.  |  Ziober, BL., et al. 1996. Semin Cancer Biol. 7: 119-28. PMID: 8773297

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Laminin β-2 Antibody (C4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Laminin β-2 Antibody (C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Laminin β-2 Antibody (C4) Specifications

Alternate Product Name: Laminin β-2

Product Clone Name: C4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Laminin β-2

Epitope Species: bovine

Product Reactivity/Detection: Laminin β-2

Species Reactivity/Detection: mouse, rat, human, bovine, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Laminin β-2 Antibody (C4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin