Laminin-5 Antibody (P3H9-2): sc-13586
- Laminin-5 Antibody (P3H9-2) is a mouse monoclonal IgG1 κ Laminin-5 antibody, cited in 16 publications, provided at 200 µg/ml
- raised against human keratinocytes expressing laminin-5
- Laminin-5 Antibody (P3H9-2) is recommended for detection of Laminin-5 of human origin by WB, IP and IF
- Anti-Laminin-5 Antibody (P3H9-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Laminin-5 Antibody (P3H9-2) for WB applications. These reagents are now offered in bundles with Laminin-5 Antibody (P3H9-2) (see ordering information below).
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Laminin-5 Antibody (P3H9-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Laminin-5 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Laminin-5 antibody (P3H9-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminin-5 is a crucial glycoprotein complex composed of three subunits: laminin-alpha3, -beta3, and -gamma2, which play a significant role in cell adhesion, signal transduction, and keratinocyte differentiation. Laminin-5 is primarily located in the basal lamina beneath epithelial tissues, where Laminin-5 is essential for anchoring basal epithelial cells to the extracellular matrix (ECM). The structural integrity and proper assembly of Laminin-5 are vital for maintaining tissue architecture and function, as any disruption can lead to pathological conditions such as blistering diseases and cancer metastasis. Furthermore, the differential processing of Laminin-5 subunits influences integration into the ECM, highlighting the importance of post-translational modifications in regulating function and interactions with other proteins.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Laminin-5 Antibody (P3H9-2) References:
- Laminin-6 is activated by proteolytic processing and regulates cellular adhesion and migration differently from laminin-5. | Hirosaki, T., et al. 2002. J Biol Chem. 277: 49287-95. PMID: 12379663
- Laminin 5 processing and its integration into the ECM. | Aumailley, M., et al. 2003. Matrix Biol. 22: 49-54. PMID: 12714041
- Laminin 5 beta3 and gamma2 chains are frequently coexpressed in cancer cells. | Akimoto, S., et al. 2004. Pathol Int. 54: 688-92. PMID: 15363037
- Laminin-5 beta3A expression in LNCaP human prostate carcinoma cells increases cell migration and tumorigenicity. | Calaluce, R., et al. 2004. Neoplasia. 6: 468-79. PMID: 15548355
- Laminin-5 is a biomarker of invasiveness in cervical adenocarcinoma. | Imura, J., et al. 2012. Diagn Pathol. 7: 105. PMID: 22898004
- Atg7 Silencing Inhibits Laminin-5 Expression to Suppress Tube Formation by Brain Endothelial Cells. | He, L., et al. 2019. Anat Rec (Hoboken). 302: 2255-2260. PMID: 31265765
- Expression of laminin-5 gamma 2 chain predicts invasion of extramammary Paget's disease cell. | Tohmatsu, Y., et al. 2021. APMIS. 129: 3-8. PMID: 32996218
- Overexpression of laminin-5 gamma-2 promotes tumorigenesis of pancreatic ductal adenocarcinoma through EGFR/ERK1/2/AKT/mTOR cascade. | Kirtonia, A., et al. 2022. Cell Mol Life Sci. 79: 362. PMID: 35699794
- Pancreatic carcinomas deposit laminin-5, preferably adhere to laminin-5, and migrate on the newly deposited basement membrane. | Tani, T., et al. 1997. Am J Pathol. 151: 1289-302. PMID: 9358755
- Laminin-5 expression is independent of the injury and the microenvironment during reepithelialization of wounds. | Kainulainen, T., et al. 1998. J Histochem Cytochem. 46: 353-60. PMID: 9487117
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin-5 Antibody (P3H9-2) | sc-13586 | 200 µg/ml | $316.00 | |||
Laminin-5 Antibody (P3H9-2): m-IgG Fc BP-HRP Bundle | sc-526564 | 200 µg Ab; 10 µg BP | $354.00 | |||
Laminin-5 Antibody (P3H9-2): m-IgG1 BP-HRP Bundle | sc-531937 | 200 µg Ab; 20 µg BP | $354.00 | |||
Laminin-5 Antibody (P3H9-2) AC | sc-13586 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Laminin-5 Antibody (P3H9-2) HRP | sc-13586 HRP | 200 µg/ml | $316.00 | |||
Laminin-5 Antibody (P3H9-2) FITC | sc-13586 FITC | 200 µg/ml | $330.00 | |||
Laminin-5 Antibody (P3H9-2) PE | sc-13586 PE | 200 µg/ml | $343.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 488 | sc-13586 AF488 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 546 | sc-13586 AF546 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 594 | sc-13586 AF594 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 647 | sc-13586 AF647 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 680 | sc-13586 AF680 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3H9-2) Alexa Fluor® 790 | sc-13586 AF790 | 200 µg/ml | $357.00 |