Laminin-5 Antibody (P3E4) recommended for detection of Laminin-5 of human origin by WB, IP and IF

Laminin-5 Antibody (P3E4): sc-13587

Laminin-5 Antibody (P3E4) is rated 5.0 out of 5 by 4.
  • y_2020, m_4, d_7, h_23
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_13587, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 157ms
  • REVIEWS, PRODUCT
5
4
4
0
3
0
2
0
1
0

Datasheets

    • Laminin-5 Antibody (P3E4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against human keratinocytes expressing Laminin-5
    • Laminin-5 Antibody (P3E4) is recommended for detection of Laminin-5 of human origin by WB, IP and IF
    • Anti-Laminin-5 Antibody (P3E4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin-5 (P3E4): sc-13587.
Submit a review for this product and receive 15 CruzCredits

What is the recommended fixation method for immunofluorescence staining with Laminin-5 (P3E4): sc-13587 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2020, m_4, d_7, h_23CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_13587, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 161ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent! This antibody is recommended for detection of Laminin-5 of human origin by WB, IP and IF
Date published: 2017-01-23
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed A-431 cells. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from IF: Publishable staining in human skin cells IF: Publishable staining in human skin cells. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by from Good IHCantibody Good IHCantibody, published using human human DM and porcine DE samples. -SCBT Publication Review
Date published: 2015-01-12
  • y_2020, m_4, d_7, h_23
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_13587, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 24ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.