Laminin-5 Antibody (P3E4): sc-13587
- Laminin-5 Antibody (P3E4) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
- raised against human keratinocytes expressing Laminin-5
- Laminin-5 Antibody (P3E4) is recommended for detection of Laminin-5 of human origin by WB, IP and IF
- Anti-Laminin-5 Antibody (P3E4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Laminin-5 Antibody (P3E4) for WB applications. This reagent is now offered in a bundle with Laminin-5 Antibody (P3E4) (see ordering information below).
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Laminin-5 Antibody (P3E4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Laminin-5 of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Laminin-5 antibody (P3E4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminin-5 is a crucial glycoprotein complex composed of three subunits: laminin-alpha3, laminin-beta3, and laminin-gamma2, which play a significant role in cell adhesion, signal transduction, and keratinocyte differentiation. Laminin-5 is primarily located in the basal lamina beneath epithelial tissues, where Laminin-5 is essential for anchoring basal epithelial cells to the extracellular matrix (ECM). The structural integrity and proper assembly of Laminin-5 are vital for maintaining tissue architecture and function, as any alterations can lead to impaired cell adhesion and contribute to various pathological conditions, including cancer metastasis and skin disorders. Furthermore, the differential processing of Laminin-5 subunits influences integration into the ECM, highlighting the importance of post-translational modifications in regulating function and interactions with other proteins.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Laminin-5 Antibody (P3E4) References:
- Deposition of laminin 5 in epidermal wounds regulates integrin signaling and adhesion. | Nguyen, BP., et al. 2000. Curr Opin Cell Biol. 12: 554-62. PMID: 10978889
- The importance of laminin 5 in the dermal-epidermal basement membrane. | Nishiyama, T., et al. 2000. J Dermatol Sci. 24 Suppl 1: S51-9. PMID: 11137397
- Laminin 5 processing and its integration into the ECM. | Aumailley, M., et al. 2003. Matrix Biol. 22: 49-54. PMID: 12714041
- Crucial role of the specificity-determining loop of the integrin beta4 subunit in the binding of cells to laminin-5 and outside-in signal transduction. | Tsuruta, D., et al. 2003. J Biol Chem. 278: 38707-14. PMID: 12867433
- Laminin-5 chains are expressed differentially in metastatic and nonmetastatic hepatocellular carcinoma. | Giannelli, G., et al. 2003. Clin Cancer Res. 9: 3684-91. PMID: 14506159
- Recombinant human laminin-5 domains. Effects of heterotrimerization, proteolytic processing, and N-glycosylation on alpha3beta1 integrin binding. | Künneken, K., et al. 2004. J Biol Chem. 279: 5184-93. PMID: 14612440
- Tumor cell alpha3beta1 integrin and vascular laminin-5 mediate pulmonary arrest and metastasis. | Wang, H., et al. 2004. J Cell Biol. 164: 935-41. PMID: 15024036
- Fibroblasts contribute to the deposition of laminin 5 in the extracellular matrix. | Elkhal, A., et al. 2004. Exp Cell Res. 296: 223-30. PMID: 15149852
- The basement membrane protein laminin-5 acts as a soluble cell motility factor. | Kariya, Y. and Miyazaki, K. 2004. Exp Cell Res. 297: 508-20. PMID: 15212952
- Microscale roughness regulates laminin-5 secretion of bone marrow mesenchymal stem cells. | Zou, J., et al. 2019. Clin Hemorheol Microcirc. 73: 237-247. PMID: 31561334
- Plasma level of laminin 5 and collagen IV in cryptorchidism. | Komarowska, M., et al. 2020. Adv Med Sci. 65: 176-181. PMID: 31978696
- Laminin-5, Fibronectin, and Type IV Collagen as Potential Biomarkers of Brain Glioma Malignancy. | Oldak, L., et al. 2022. Biomedicines. 10: PMID: 36140391
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin-5 Antibody (P3E4) | sc-13587 | 200 µg/ml | $316.00 | |||
Laminin-5 Antibody (P3E4): m-IgG1 BP-HRP Bundle | sc-531639 | 200 µg Ab; 20 µg BP | $354.00 | |||
Laminin-5 Antibody (P3E4) AC | sc-13587 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Laminin-5 Antibody (P3E4) HRP | sc-13587 HRP | 200 µg/ml | $316.00 | |||
Laminin-5 Antibody (P3E4) FITC | sc-13587 FITC | 200 µg/ml | $330.00 | |||
Laminin-5 Antibody (P3E4) PE | sc-13587 PE | 200 µg/ml | $343.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 488 | sc-13587 AF488 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 546 | sc-13587 AF546 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 594 | sc-13587 AF594 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 647 | sc-13587 AF647 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 680 | sc-13587 AF680 | 200 µg/ml | $357.00 | |||
Laminin-5 Antibody (P3E4) Alexa Fluor® 790 | sc-13587 AF790 | 200 µg/ml | $357.00 |